Use of rotavirus virus-like particles as surrogates to evaluate virus persistence in shellfish

Type Article
Date 2005-10
Language English
Author(s) Loisy Fabienne1, Atmar R2, Le Saux Jean-Claude1, Cohen J3, Caprais Marie-Paule1, Pommepuy Monique1, Le Guyader Soizick1
Affiliation(s) 1 : IFREMER, Microbiol Lab, F-44311 Nantes, France.
2 : Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA.
3 : CNRS, INRA, Unite Mixte, F-91198 Gif Sur Yvette, France.
Source Applied and environmental microbiology (0099-2240) (American society for microbiology), 2005-10 , Vol. 71 , N. 10 , P. 6049-6053
DOI 10.1128/AEM.71.10.6049-6053.2005
WOS© Times Cited 53
Keyword(s) Seawater, Viral diseases, Bioaccumulation, Depuration, Contamination, Model, Pancreas, Regression analysis, Shellfish, Phages, Virus
Abstract Rotavirus virus-like particles (VLPs) and MS2 bacteriophages were bioaccumulated in bivalve mollusks to evaluate viral persistence in shellfish during depuration and relaying under natural conditions. Using this nonpathogenic surrogate virus, we were able to demonstrate that about 1 log(10) of VLPs was depurated after 1 week in warm seawater (22 degrees C). Phage MS2 was depurated more rapidly (about 2 log(10) in 1 week) than were VLPs, as determined using a single-compartment model and linear regression analysis. After being relayed in the estuary under the influence of the tides, VLPs were detected in oysters for up to 82 days following seeding with high levels of VLPs (concentration range between 10(10) and 10(9) particles per g of pancreatic tissue) and for 37 days for lower contamination levels (10(5) particles per g of pancreatic tissue). These data suggest that viral particles may persist in shellfish tissues for several weeks.
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