Oligonucleotide quartz crystal microbalance sensor for the microalgae Alexandrium minutum (Dinophyceae)
|Author(s)||Lazerges M1, Perrot H1, Antoine Elisabeth2, Defontaine Audrey2, Compere Chantal3|
|Affiliation(s)||1 : Univ Paris 06, CNRS, LISE, F-75252 Paris 05, France.
2 : IFREMER, Ctr Nantes, F-44311 Nantes, France.
3 : IFREMER, Ctr Brest, F-29280 Plouzane, France.
|Source||Biosensors and Bioelectronics (0956-5663) (Elsevier), 2006 , Vol. 21 , N. 7 , P. 1355-1358|
|WOS© Times Cited||22|
|Keyword(s)||DNA, Alexandrium minutum, Microbalance|
|Abstract||We report the immobilization on a gold surface of a 20-base DNA probe labeled with disulfide group and on the selective hybridization with the complementary 20-base DNA strand. The oligonucleotide probe is the complementary strand of a partial sequence of the gene encoding for a large ribosomal RNA sub-unit which is a coding sequence of Alexandrium minutum DNA, a microalgae that produces neurotoxins responsible for paralytic shellfish poisoning on European and Asian coasts. The kinetics of DNA probe immobilization and hybridization were monitored in situ by using a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the setup is stable to within a few hertz, corresponding to the nanogram, scale, for 3 h and makes it possible to follow frequency change from immobilization of the probe to hybridization of the complementary DNA target. This setup constitutes a biosensor, which is sensitive and selective, and the hybridization ratio between hybridized complementary DNA and immobilized DNA probes is 47%.|