Purification and characterization of infectious myonecrosis virus of penaeid shrimp

Type Article
Date 2006-04
Language English
Author(s) Poulos Bonnie1, Tang K1, Pantoja C1, Bonami Jean-Robert2, 3, Lightner D1
Affiliation(s) 1 : Univ Arizona, Aquaculture Pathol Lab, Dept Vet Sci & Microbiol, Tucson, AZ 85721 USA.
2 : CNRS, IFREMER, UM2, UMR 5098, F-34095 Montpellier 5, France.
Source JOURNAL-OF-GENERAL-VIROLOGY (0022-1317) (Journal of General Virology), 2006-04 , Vol. 87 , P. 987-996
DOI 10.1099/vir.0.81127-0
WOS© Times Cited 118
Keyword(s) Purification, Myonecrosis, Virus, Shrimp, Penaeid
Abstract The causative agent of myonecrosis affecting cultured Penaeus vannamei in Brazil was demonstrated to be a virus after purification of the agent from infected shrimp tissues. Purified viral particles were injected into specific pathogen-free P. vannamei, resulting in a disease that displayed the same characteristics as those found in the original shrimp used for purification. The virus was named infectious myonecrosis virus (IMNV). The viral particles were icosahedral in shape and 40 nm in diameter, with a buoyant density of 1(.)366 g ml(-1) in caesium chloride. The genome consisted of a single, double-stranded (dsRNA) molecule of 7560 bp. Sequencing of the viral genome revealed two non-overlapping open reading frames (ORFs). The 5' ORF (ORF 1, nt 136-4953) encoded a putative RNA-binding protein and a capsid protein. The coding region of the RNA-binding protein was located in the first half of ORF 1 and contained a dsRNA-binding motif in the first 60 aa. The second half of ORF 1 encoded a capsid protein, as determined by amino acid sequencing, with a molecular mass of 106 kDa. The 3' ORF (ORF 2, nt 5241-7451) encoded a putative RNA-dependent RNA polymerase (RdRp) with motifs characteristic of totiviruses. Phylogenetic analysis based on the RdRp clustered IMNV with Giardia lamblia virus, a member of the family Totiviridae. Based on these findings, IMNV may be unique member of the Totiviridae or may represent a new dsRNA virus family that infects invertebrate hosts.
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