Improvement of lipid and phospholipid recoveries from sardine (Sardina pilchardus) viscera using industrial proteases

Type Article
Date 2006-11
Language English
Author(s) Dumay Justine1, Donnay-Moreno Claire1, Barnathan G2, Jaouen P3, Berge Jean-PascalORCID1
Affiliation(s) 1 : IFREMER, F-44311 Nantes, France.
2 : Univ Nantes, Nantes Atlant Univ, SMAB, Fac Pharm, F-44035 Nantes, France.
3 : CNRS, UMR 6144, GEPEA, Samt Nazaire, France.
Source Process Biochemistry (1359-5113) (Elsevier), 2006-11 , Vol. 41 , N. 11 , P. 2327-2332
DOI 10.1016/j.procbio.2006.04.005
WOS© Times Cited 56
Keyword(s) Fish protein hydrolysate FPH, Sardine, By product, Lipid, Hydrolysis, Phospholipid
Abstract Enzymatic hydrolysis of sardine viscera by three broad spectrum proteases was investigated using the pHstat method (24 h, pH 8, 50 degrees C). After hydrolysis, three fractions (sludge, aqueous phase and oily phase) were collected. For each fraction, lipids and phospholipids were quantified and molecular weights of aqueous phase peptides were determined. Under these conditions, the degree of hydrolysis (DH) varied from 1.9% (Flavourzyme), 3.1% (Protamex) and to 3.3% (Alcalase). Dry matter distribution indicated that all hydrolysis performed increased solubilisation of proteins: protein recovery for each hydrolysis ranged from 57.4% to 61.2%. Furthermore, the total lipid content in the liquid parts (oily and aqueous phases) increased (at least 85% of lipids quantified in the raw material are in these phases) which may improve lipid recovery for commercial applications. In addition, these lipids were richer in phospholipids than those extracted by classical chemical extraction, especially after Alcalase hydrolysis. (c) 2006 Elsevier Ltd. All rights reserved.
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