Monoclonal antibodies against Bonamia ostreae (Protozoa: Ascetospera), an intrahaemocytic parasite of flat oyster Ostrea edulis (Mollusca : Bivlavia)
| Type | Article | ||||||||
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| Date | 1991-08 | ||||||||
| Language | English | ||||||||
| Author(s) | Rogier Hervé1, Hervio Dominique2, Boulo Viviane 2, Clavies C1, Hervaud E1, Bachere Evelyne2, Mialhe Eric2, Grizel Henri2, Pau B3, Paolucci F1 |
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| Affiliation(s) | 1 : Sanofi Recherche, Rue du Professeur, Blayac, F-34082 Montpellier, France 2 : IFREMER, Unite de Recherches en Pathologie, Immunologie et Genetique Moleculaire (URPIGM), B.P. 133, F-17390 La Tremblade, France 3 : Faculte de Pharmacie, Avenue Charles Flahault, F-34060 Montpellier Cedex, France |
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| Source | Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 1991-08 , Vol. 11 , N. 2 , P. 135-142 | ||||||||
| DOI | 10.3354/dao011135 | ||||||||
| WOS© Times Cited | 19 | ||||||||
| Abstract | The protozoan Bonamia ostreae (Ascetospora), a paasite of the flat oyster Ostrea edulis, was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibiodies identified. One of these antibodies (20B2-1B12) has an apparent association constant of ca 3 X 10(8) M-1 and bound to a number of different sites on the parasite. These monoclonal antibodies should prove to be of great value as diagnostic and research tools. | ||||||||
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