Preparation and characterization of monoclonal antibodies against neoplastic hemocytes of Mytilus edulis (Bivalvia)
|Author(s)||Noel Danielle1, Boulo Viviane1, Chagot Dominique1, Mialhe Eric1, Paolucci Francis2, Clavies Christine2, Hervaud Eric2, Elston Ralph3|
|Affiliation(s)||1 : IFREMER, PATHOL & GENET INVERTEBRES MARINS LAB, BP 133, F-17390 LA TREMBLADE, FRANCE.
2 : SANOFI, Service Immunodiagnostic, rue du Professeur Blayac, F-34082 Montpellier, France
3 : Battelle, Marine Sciences Laboratory, 439 W. Sequim Bay Road, Sequim, Washington 98382, USA
|Source||Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 1991-03 , Vol. 10 , N. 1 , P. 51-58|
|WOS© Times Cited||20|
|Abstract||Mytilus edulis develop progressive and fatal hemocytic neoplasia. We have generated monoclonal antibodies using neoplastic hemocytes from highly infected individuals collected from Puget Sound in Washington State (USA). Twenty-seven monoclonal antibodies against neoplatic cell antigens were obtained. Specificity of 2 purified antibodies, 14F1 and 16G10, was demonstrated by immunofluorescence when incubated with neoplastic hemocytes, whereas these anbidodies did not react with normal cells. This specificity was confirmed by immunogold labelling and electron microscopic visualization. The technique permitted localization of the epitopes recognized by the monoclonal antibodies on the plasma membrane. These monoclonal antibodies are suitable reagents for establishment of immunoassays for diagnosis and quantification of hemic neoplasia in the mussel.|