Needle in a haystack: involvement of the copepod Paracartia grani, in the life-cycle of the oyster pathogen Marteilia refringens

Type Article
Date 2002-09
Language English
Author(s) Audemard Corinne, Le Roux Frédérique, Barnaud Antoine, Collins Catherine, Sautour B, Sauriau Pierre-Guy, De Montaudouin X, Coustau C, Combes C, Berthe Franck
Affiliation(s) IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
Univ Bordeaux 1, UMR 5805, Lab Oceanog Biol, CNRS 2, F-33120 Arcachon, France.
Ctr Rech Ecol Marine & Aquaculture, F-17137 Houmeau, France.
Univ Perpignan, CBETM, CNRS, UMR 5555 Lab Biol Anim, F-66860 Perpignan, France.
Source Parasitology (0031-1820) (Cambridge University Press), 2002-09 , Vol. 124 , P. 315-323
DOI 10.1017/S0031182001001111
WOS© Times Cited 65
Keyword(s) Experimental transmission, In situ hybridization, PCR, Paracartia grani, Ostrea edulis, Marteilia refringens
Abstract Marteilia refringens is a major pathogen of the European flat oyster, Ostrea edulis Linnaeus. Since its description, the lifecycle of this protozoan parasite has eluded discovery. Attempts to infect oysters experimentally have been unsuccessful and led to the hypothesis of a complex life-cycle involving several hosts. Knowledge of this life-cycle is of central importance in order to manage oyster disease. However, the exploration of M. refringens life-cycle has been previously limited by the detection tools available and the tremendous number of species to be screened in enzootic areas. In this study, these two restrictions were circumvented by the use of both molecular detection tools and a mesocosm with low biodiversity. Screening of the entire fauna of the pond for AT. refringens DNA was systematically undertaken using PCR. Here, we show that the copepod Paracartia (Acartia) grani is a host of M. refringens. Not only was DNA of M. refringens consistently detectcd in P. grani but also the presence of the parasite in the ovarian tissues was demonstrated using in situ hybridization. Finally, successful experimental transmissions provided evidence that P. grani can be infected from infected flat oysters.
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Audemard Corinne, Le Roux Frédérique, Barnaud Antoine, Collins Catherine, Sautour B, Sauriau Pierre-Guy, De Montaudouin X, Coustau C, Combes C, Berthe Franck (2002). Needle in a haystack: involvement of the copepod Paracartia grani, in the life-cycle of the oyster pathogen Marteilia refringens. Parasitology, 124, 315-323. Publisher's official version : https://doi.org/10.1017/S0031182001001111 , Open Access version : https://archimer.ifremer.fr/doc/00000/2778/