Screening of Vibrio isolates to develop an experimental infection model in the Pacific oyster Crassostrea gigas

Type Article
Date 2004-04
Language English
Author(s) Gay Melanie, Berthe Franck, Leroux Frederique
Affiliation(s) IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
Source Diseases of aquatic organisms (0177-5103) (Inter-Research), 2004-04 , Vol. 59 , N. 1 , P. 49-56
WOS© Times Cited 93
Keyword(s) Crassostrea gias, Antagonism, Agonism, Virulence, Vibrio splendidus
Abstract In an attempt to develop a reproducible experimental model of bacterial infection in Crassostrea gigas, oysters taken from very localised sub-populations suffering natural mortality outbreaks were used in cohabitation trials under laboratory conditions. From these trials, a collection of Vibrio strains was isolated from moribund and healthy oysters. In a second step, strains were experimentally tested for virulence by means of injection into healthy oysters. This screening revealed a span of virulence among isolated strains from none to medium. When pooling injected strains, results suggest increased virulence. Vibrio strains may have additive/synergistic action leading to higher C, gigas mortality rates in experimental challenges. Although the study initially aimed to develop a simple experimental model, a complex of interactions emerged between several bacterial strains during the pathogenic process in their molluscan host. Selected strains provide a suitable model of experimental disease for further studies and better understanding of bacterial interaction and pathogenesis in C. gigas.
Full Text
File Pages Size Access
publication-3174.pdf 8 202 KB Open access
Top of the page

How to cite 

Gay Melanie, Berthe Franck, Leroux Frederique (2004). Screening of Vibrio isolates to develop an experimental infection model in the Pacific oyster Crassostrea gigas. Diseases of aquatic organisms, 59(1), 49-56. Open Access version : https://archimer.ifremer.fr/doc/00000/3174/