||Barracco Margherita1, 2, De Lorgeril Julien1, Gueguen Yannick1, Bachere Evelyne1
||1 : Univ Montpellier 2, IFREMER, CNRS, UMR Genome Populat Interact Adaptat 5171, F-34095 Montpellier, France.
2 : Univ Fed Santa Catarina, Dept Biol Celular Embriol & Genet, Florianopolis, SC, Brazil.
||FEMS Microbiology Letters (0378-1097) (Blackwell science), 2005-09 , Vol. 250 , N. 1 , P. 117-120
|WOS© Times Cited
||Immuno detection, Molecular cloning, Litopenaeus schmitti, Farfantepenaeus paulensis, Brazilian penaeid shrimps, Hemocytes, Antimicrobial peptides, Penaeidins
||We report here the molecular cloning of new members of the penaeidin family from two Atlantic penaeids from Brazil, Litopenaeus schmitti and Farfantepenaeus paulensis. The presence of penaeidins in the granular hemocytes of both shrimps was first evidenced by immunofluorescence, using polyclonal antibodies raised against L. vannamei penaeidin Litvan PEN3-1. cDNAs from the hemocytes of both Brazilian species were obtained by reverse transcription and the sequences encoding penaeidins were amplified by PCR, using primers based on penaeidin consensus sequences. Five penaeidin clones were obtained. According to the international penaeidin classification (PenBase, http://www.penbase.immunaqua.com), the deduced amino acid sequences of two clones from L. schmitti and two from F. paulensis belong to the PEN2 subgroup and one clone from L. schmitti to the PEN4 subgroup of penaeidins. Surprisingly, no penacidin from the PEN3 subgroup was obtained in both shrimp species, even though this subgroup appears to be the most commonly expressed in the hemocytes of penaeids. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.