A novel mcl PHA-producing bacterium, Pseudomonas guezennei sp nov, isolated from a 'kopara' mat located in Rangiroa, an atoll of French Polynesia

Type Article
Date 2008-02
Language English
Author(s) Simon-Colin Christelle1, Alain Karine2, Colin Sebastien1, Cozien Joelle1, Costa B, Guezennec Jean1, Raguenes Gerard1
Affiliation(s) 1 : IFREMER, Ctr Brest, BIOMAR BMM, F-29280 Plouzane, France.
2 : Equipe Ecol Microbienne Mrine, Lab Oceanog Biol Banyuls, Banyuls Sur Mer, France.
Source Journal of Applied Microbiology (1364-5072) (Blackwell science), 2008-02 , Vol. 104 , N. 2 , P. 581-586
DOI 10.1111/j.1365-2672.2007.03568.x
WOS© Times Cited 10
Keyword(s) Pseudomonas, Polyhydroxyalkanaote, Microbial mat, Kopara
Abstract Aims: The aim of the present study was to describe an aerobic, mesophilic and heterotrophic bacterium, designated RA26, able to produce a medium-chain-length polyhydroxyalkanoate (PHA). It was isolated from a French Polynesian bacterial mat located in the atoll of Rangiroa.

Methods and Results: This micro-organism, on the basis of the phenotypical features and genotypic investigations can be clearly assigned to the Pseudomonas genus and the name of Pseudomonas guezennei is proposed. Optimal growth occurs between 33 and 37 degrees C, at a pH between 6 4 and 7 1 and at ionic strength of 15 g 1(-1) of sea salts. The G+C content of DNA is 63.2%. Under laboratory conditions, this bacterium produced a novel, medium-chain-length PHA, mainly composed of 3-hydroxydecanaote ( 64 mol.%) and 3-hydroxyoctanoate ( 24 mol.%) ( GC-MS, NMR) from a single nonrelated carbon substrate, i.e. glucose.

Conclusions: The bacterium P. guezennei produces a novel PHA mcl with elastomeric properties.

Significance and Impact of the Study: PHAs share physical and material properties that recommend them for application in various areas, and are considered as an alternative to nonbiodegradable plastics produced from fossil oils. In this study, we describe a new bacteria with the capability to synthesize a novel PHA with promising biotechnological applications.
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Simon-Colin Christelle, Alain Karine, Colin Sebastien, Cozien Joelle, Costa B, Guezennec Jean, Raguenes Gerard (2008). A novel mcl PHA-producing bacterium, Pseudomonas guezennei sp nov, isolated from a 'kopara' mat located in Rangiroa, an atoll of French Polynesia. Journal of Applied Microbiology, 104(2), 581-586. Publisher's official version : https://doi.org/10.1111/j.1365-2672.2007.03568.x , Open Access version : https://archimer.ifremer.fr/doc/00000/3858/