Characterization of a gonad-specific transforming growth factor-beta superfamily member differentially expressed during the reproductive cycle of the oyster Crassostrea gigas

Type Article
Date 2008-02
Language English
Author(s) Fleury ElodieORCID1, 2, Fabioux Caroline1, 2, Lelong C2, Favrel P2, Huvet ArnaudORCID1
Affiliation(s) 1 : IFREMER, Ctr Brest, UMR M100 PE2M, F-29280 Plouzane, France.
2 : Univ Caen, UMR M100 PE2M, F-14032 Caen, France.
Source Gene (0378-1119) (Elsevier), 2008-02 , Vol. 410 , N. 1 , P. 187-196
DOI 10.1016/j.gene.2007.12.017
WOS© Times Cited 27
Keyword(s) Reproduction, Oyster, Gene expression, Transforming growth factor, Bivalve mollusc
Abstract Through differential screening between oyster families selected for high and low summer survival, we have characterized a new transforming growth factor-beta (TGF-beta) superfamily member. This novel factor, named oyster-gonadal-TGF beta-like (og-TGF beta-like), is synthesized as a 307 amino acid precursor and displays 6 of the 7 characteristic cysteine residues of the C-terminal, mature peptide. Sequence comparison revealed that og-TGF beta-like has a low percentage of identity with other known TGF-beta superfamily members, suggesting that og-TGF beta-like is a derived member of this large superfamily. Real-time PCR (RT-PCR) analysis in different oyster tissues showed that og-TGF beta-like is specifically expressed in both male and female gonads, at distinct levels according to the reproductive stage. Og-TGF beta-like relative expression was the lowest at the initiation of the reproductive cycle and increased as maturation proceeded to achieve a maximal level in fully mature female and male oysters. In situ hybridisation demonstrated that expression was exclusively detected in the somatic cells surrounding oocytes and spermatocytes. The role of this newly-characterized TGF beta member in the reproduction of cupped oyster is discussed in regard to the specificity and the localization of its expression, which singularly contrasts with the pleiotropic roles in a variety of physiological processes commonly ascribed to most TGF-beta family members identified so far. (C) 2007 Elsevier B.V. All rights reserved.
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Fleury Elodie, Fabioux Caroline, Lelong C, Favrel P, Huvet Arnaud (2008). Characterization of a gonad-specific transforming growth factor-beta superfamily member differentially expressed during the reproductive cycle of the oyster Crassostrea gigas. Gene, 410(1), 187-196. Publisher's official version : https://doi.org/10.1016/j.gene.2007.12.017 , Open Access version : https://archimer.ifremer.fr/doc/00000/3899/