Penaeidins, antimicrobial peptides with chitin-binding activity, are produced and stored in shrimp granulocytes and released after microbial challenge

Type Article
Date 2000-02
Language English
Author(s) Destoumieux-Garzon Delphine, Munoz Marcello, Cosseau Céline, Rodriguez Jenny, Bulet Philippe, Comps Marie-Annick, Bachere Evelyne
Affiliation(s) Univ Montpellier 2, IFREMER, JCSCNRS, UMR 219, F-34095 Montpellier, France.
CNRS, UPR 9022, Inst Biol Mol & Cellulaire, F-67084 Strasbourg, France.
Escuela Super Politecn Litoral, Ctr Nacl Acuicultura & Invest Marinas, Guayaquil, Ecuador.
IFREMER, F-34230 Palavas Les Flots, France.
Source Journal of Cell Science (0021-9533) (Company of Biologists), 2000-02 , Vol. 113 , N. 3 , P. 461-469
WOS© Times Cited 191
Keyword(s) Crustacean immunity, Penaeid shrimp, Haemocyte, Chitin binding, Antimicrobial peptide
Abstract Penaeidins are members of a new family of antimicrobial peptides isolated from a crustacean, which present both Gram-positive antibacterial and antifungal activities. We have studied the localization of synthesis and storage of penaeidins in the shrimp Penaeus vannamei. The distribution of penaeidin transcripts and peptides in various tissues reveals that penaeidins are constitutively synthesized and stored in the shrimp haemocytes. It was shown by immunocytochemistry, at both optical and ultrastructural levels, that the peptides are localized in granulocyte cytoplasmic granules, The expression and localization of penaeidins were further analysed in shrimp subjected to microbial challenge. We found that (1) penaeidin mRNA levels decrease in circulating haemocytes in the first 3 hours following stimulation and (2) an increase in plasma penaeidin concentration occurs after microbial challenge, together with (3) a penaeidin immunoreactivity in cuticular tissue, which can be related to the chitin-binding activity we demonstrate here for penaeidins.
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Destoumieux-Garzon Delphine, Munoz Marcello, Cosseau Céline, Rodriguez Jenny, Bulet Philippe, Comps Marie-Annick, Bachere Evelyne (2000). Penaeidins, antimicrobial peptides with chitin-binding activity, are produced and stored in shrimp granulocytes and released after microbial challenge. Journal of Cell Science, 113(3), 461-469. Open Access version : https://archimer.ifremer.fr/doc/00000/412/