Purification and partial identification of novel antimicrobial protein from marine bacterium Pseudoalteromonas species strain x 4 53
|Author(s)||Longeon Arlette, Peduzzi Jean, Barthelemy Michel, Corre Sophie, Nicolas Jean-Louis, Guyot Museum National D'Histoire Naturelle|
|Affiliation(s)||CNRS, UMR 5154, Natl Museum Nat Hist, Lab Chim & Biochim Substances Nat,USM 502, F-75005 Paris, France.
Micromer Technopole Brest Iroise, F-29200 Brest, France.
IFREMER, DRV, Lab Physiol Invertebres, F-29280 Plouzane, France.
|Source||Marine Biotechnology (1436-2228) (Springer), 2004-11 , Vol. 6 , N. 6 , P. 633-641|
|WOS© Times Cited||56|
|Keyword(s)||Probiotic bacteria, Pseudoalteromonas, Antimicrobial protein|
|Abstract||A marine bacterium, X 153, was isolated from a pebble collected at St. Anne du Portzic (France). By 16S ribosomal DNA gene sequence analysis, X153 strain was identified as a Pseudoalteromonas sp. close to P. piscicida. The crude culture of X153 was highly active against human pathogenic strains involved in dermatologic diseases, and marine bacteria including various ichthyopathogenic Vibrio strains. The active substance occurred both in bacterial cells and in culture supernatant. An antimicrobial protein was purified to homogeneity by a 4-step procedure using size-exclusion and ion-exchange chromatography. The highly purified P-153 protein is anionic, and sodium dodecylsulfate polyacrylamide gel electrophoresis gives all apparent molecular mass of 87 kDa. The X153 bacterium protected bivalve larvae against mortality, following experimental challenges with ichthyopathogenic Vibrio. Pseudoalteromonas sp. X153 may be useful in aquaculture as a probiotic bacterium.|