Purification and partial identification of novel antimicrobial protein from marine bacterium Pseudoalteromonas species strain x 4 53

Type Article
Date 2004-11
Language English
Author(s) Longeon Arlette, Peduzzi Jean, Barthelemy Michel, Corre Sophie, Nicolas Jean-Louis, Guyot Museum National D'Histoire Naturelle
Affiliation(s) CNRS, UMR 5154, Natl Museum Nat Hist, Lab Chim & Biochim Substances Nat,USM 502, F-75005 Paris, France.
Micromer Technopole Brest Iroise, F-29200 Brest, France.
IFREMER, DRV, Lab Physiol Invertebres, F-29280 Plouzane, France.
Source Marine Biotechnology (1436-2228) (Springer), 2004-11 , Vol. 6 , N. 6 , P. 633-641
DOI 10.1007/s10126-004-3009-1
WOS© Times Cited 55
Keyword(s) Probiotic bacteria, Pseudoalteromonas, Antimicrobial protein
Abstract A marine bacterium, X 153, was isolated from a pebble collected at St. Anne du Portzic (France). By 16S ribosomal DNA gene sequence analysis, X153 strain was identified as a Pseudoalteromonas sp. close to P. piscicida. The crude culture of X153 was highly active against human pathogenic strains involved in dermatologic diseases, and marine bacteria including various ichthyopathogenic Vibrio strains. The active substance occurred both in bacterial cells and in culture supernatant. An antimicrobial protein was purified to homogeneity by a 4-step procedure using size-exclusion and ion-exchange chromatography. The highly purified P-153 protein is anionic, and sodium dodecylsulfate polyacrylamide gel electrophoresis gives all apparent molecular mass of 87 kDa. The X153 bacterium protected bivalve larvae against mortality, following experimental challenges with ichthyopathogenic Vibrio. Pseudoalteromonas sp. X153 may be useful in aquaculture as a probiotic bacterium.
Full Text
File Pages Size Access
publication-426.pdf 22 176 KB Open access
Top of the page

How to cite 

Longeon Arlette, Peduzzi Jean, Barthelemy Michel, Corre Sophie, Nicolas Jean-Louis, Guyot Museum National D'Histoire Naturelle (2004). Purification and partial identification of novel antimicrobial protein from marine bacterium Pseudoalteromonas species strain x 4 53. Marine Biotechnology, 6(6), 633-641. Publisher's official version : https://doi.org/10.1007/s10126-004-3009-1 , Open Access version : https://archimer.ifremer.fr/doc/00000/426/