First report of a Mikrocytos-like parasite in European oysters Ostrea edulis from Canada after transport and quarantine in France
|Author(s)||Gagne N1, Cochennec Nathalie3, Stephenson M1, McGladdery S2, Meyer G4, Bower S4|
|Affiliation(s)||1 : Fisheries & Oceans Canada, Gulf Fisheries Ctr, Moncton, NB E1C 9B6, Canada.
2 : Fisheries & Oceans Canada, Natl Capital Reg, Ottawa, ON K1A 0E6, Canada.
3 : IFREMER, Ctr Pacifique, F-98719 Taravao, Tahiti, Fr Polynesia.
4 : Fisheries & Oceans Canada, Pacific Biol Stn, Nanaimo, BC V9R 5K6, Canada.
|Source||Diseases of aquatic organisms (depuis 2001) (0177-5103) (Inter-Research), 2008-06 , Vol. 80 , N. 1 , P. 27-35|
|WOS© Times Cited||19|
|Keyword(s)||Histology, PCR, In situ hybridization, Ostrea edulis, Mikrocytos|
|Abstract||As part of a disease resistance experiment, 112 apparently healthy European flat oysters Ostrea edulis L. were exported from Canada (Nova Scotia) into France to test their susceptibility to Bonamia ostreae infection. Twelve oysters died in transit and 17 others died within 2 wk of laboratory quarantine acclimation. All oysters were examined histologically, and the 17 that died during quarantine were assayed for microcells (Bonamia sp. and Mikrocytos mackini) using molecular techniques. A microcell parasite was detected in the connective tissue of 5 of the 112 oysters. Morphological appearance, tissue affinity and molecular characterization through PCR, in situ hybridization (ISH), fluorescence in situ hybridization (FISH) and sequencing revealed a protist related to M mackini. This is the first report of a parasite of the genus Mikrocytos in a species belonging to the genus, Ostrea from the Atlantic Ocean.|