Shewanella profunda sp nov., isolated from deep marine sediment of the Nankai Trough

Type Article
Date 2004-11
Language English
Author(s) Toffin Laurent, Bidault Adeline, Pignet Patricia, Tindall Brian, Slobodkin Alexander, Kato Chiaki, Prieur Daniel
Affiliation(s) CNRS, UMR 6539, F-29280 Plouzane, France.
Univ Bretagne Occidentale, Technopole Brest Iroise, F-29280 Plouzane, France.
Japan Marine Sci & Technol Ctr, Marine Ecosyst Res Dept, Yokosuka, Kanagawa 2370061, Japan.
IFREMER, Ctr Brest, Dept Valorisat Prod, Lab Microbiol & Biotechnol Extremophiles, F-29280 Plouzane, France.
Deutsch Sammlung Mikroorganismen & Zellkulturen, D-38124 Braunschweig, Germany.
Russian Acad Sci, Inst Microbiol, Moscow 117312, Russia.
Source International journal of systematic and evolutionary microbiology (1466-5026) (Society for General Microbiology), 2004-11 , Vol. 54 , N. 6 , P. 1943-1949
DOI 10.1099/ijs.0.03007-0
WOS© Times Cited 52
Keyword(s) Phylogeny, Bacteria, Microbiology, Deep sediment layer
Abstract A novel piezotolerant, mesophilic, facultatively anaerobic, organotrophic, polarly flagellated bacterium (strain LT13a(T)) was isolated from a deep sediment layer in the Nankai Trough (Leg 190, Ocean Drilling Program) off the coast of Japan. This organism used a wide range of organic substrates as sole carbon and energy sources: pyruvate, glutamate, succinate, fumarate, lactate, citrate, peptone and tryptone. Oxygen, nitrate, fumarate, ferric iron and cystine were used as electron acceptors. Maximal growth rates were observed at a hydrostatic pressure of 10 MPa. Hydrostatic pressure for growth was in the range 0.1-50 MPa. Predominant cellular fatty acids were 16:1 omega7c, 15: 0 iso, 16: 0 and 13: 0 iso. The G + C content of the DNA was 44.9 mol%. On the basis of 16S rRNA gene sequences, strain LT13a(T) was shown to belong to the gamma-Proteobacteria, being closely related to Shewanella putrefaciens (98%), Shewanella oneidensis (97%) and Shewanella baltica (96 %). Levels of DNA homology between strain LT13a(T) and S. putrefaciens, S. oneidensis and S. baltica were < 20 %, indicating that strain LT13a(T) represents a novel species. Genetic evidence and phenotypic characteristics showed that isolate LT13a(T) constitutes a novel species of the genus Shewanella, Because of the deep origin of the strain, the name Shewanella profunda sp. nov. is proposed, with LT13a(T) (= DSM 15900(T)-JCM 12080(T)) as the type strain.
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Toffin Laurent, Bidault Adeline, Pignet Patricia, Tindall Brian, Slobodkin Alexander, Kato Chiaki, Prieur Daniel (2004). Shewanella profunda sp nov., isolated from deep marine sediment of the Nankai Trough. International journal of systematic and evolutionary microbiology, 54(6), 1943-1949. Publisher's official version : https://doi.org/10.1099/ijs.0.03007-0 , Open Access version : https://archimer.ifremer.fr/doc/00000/590/