Oyster hemocytes express a proline-rich peptide displaying synergistic antimicrobial activity with a defensin

Type Article
Date 2009-02
Language English
Author(s) Gueguen YannickORCID1, Romestand Bernard1, Fievet Julie1, Schmitt Paulina1, Destoumieux Garzon Delphine1, Franck Vandenbulcke2, Philippe Bulet3, Bachere Evelyne1
Affiliation(s) 1 : Univ Montpellier 2, CNRS, Ifremer, UMR Ecosyst Lagunaires 5119, F-34095 Montpellier 5, France.
2 : Univ Lille 1, EA 3570, Lab Ecol Numer & Ecotoxicol, F-59655 Villeneuve Dascq, France.
3 : UJF, CNRS, TIMC IMAG, UMR 5525, F-74160 Archamps, France.
Source Molecular Immunology (0161-5890) (Elsevier), 2009-02 , Vol. 46 , N. 4 , P. 516-522
DOI 10.1016/j.molimm.2008.07.021
WOS© Times Cited 66
Keyword(s) Antimicrobial peptide, Mollusk, Synergy, Invertebrate, Innate immunity, Pacific oyster
Abstract A cDNA sequence that encodes a 61-amino acid polypeptide precursor with homologies to proline-rich antimicrobial peptides (AMPs) was identified in the oyster Crassostrea gigas. After release of a hydrophobic signal peptide. the resulting 37-amino acid peptide, Cg-Prp, is composed of an acidic region and a cationic proline-rich region. To evaluate the biological properties of Cg-Prp, multiple proline-rich peptides corresponding to putative processing of the full-length Cg-Prp were synthesized. A limited antimicrobial activity was observed for two of them, which also showed strong synergistic antimicrobial activity with Cg-Def, a defensin from C gigas. To our knowledge, this is the first evidence of synergy between a defensin and another AMP in an invertebrate. By in situ hybridization, the expression of Cg-prp was found to be restricted to hemocytes and induced following bacterial challenge. Cg-prp transcripts were also detected in hemocytes infiltrating mantle, where Cg-Def is expressed. Additionally, by immunocytochemistry, we showed that Cg-Prp or one of its variants is present in some hemocytes together with defensins. In conclusion, we described here the first proline-rich AMP from mollusk. From our study, it is likely to provide a first line of defense against bacterial invasion by acting through synergy with defensins. (C) 2008 Elsevier Ltd. All rights reserved.
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Gueguen Yannick, Romestand Bernard, Fievet Julie, Schmitt Paulina, Destoumieux Garzon Delphine, Franck Vandenbulcke, Philippe Bulet, Bachere Evelyne (2009). Oyster hemocytes express a proline-rich peptide displaying synergistic antimicrobial activity with a defensin. Molecular Immunology, 46(4), 516-522. Publisher's official version : https://doi.org/10.1016/j.molimm.2008.07.021 , Open Access version : https://archimer.ifremer.fr/doc/00000/6232/