Rapid enzyme-linked immunosorbent assay for detection of the algal toxin domoic acid

Type Article
Date 2008-12
Language English
Author(s) Litaker R. Wayne1, Stewart Thomas N.2, Eberhart Bich-Thuy L.3, Wekell John C.3, Trainer Vera L.3, Kudela Raphael M.4, Miller Peter E.4, Roberts Alice4, Hertz Cassandra4, Johnson Tyler A.4, Frankfurter Greg5, Smith G. Jason5, Schnetzer Astrid6, Schumacker Joe7, Bastian Jonnette L.7, Odell Anthony8, Gentien Patrick9, Le Gal Dominique10, Hardison I. Ransom1, Tester Patricia A.1
Affiliation(s) 1 : NOAA, Natl Ocean Serv, Beaufort, NC 28516 USA.
2 : Mercury Sci Inc, Raleigh, NC 27607 USA.
3 : NOAA, NW Fisheries Sci Ctr, Seattle, WA 98112 USA.
4 : Univ Calif Santa Cruz, Inst Marine Sci, Santa Cruz, CA 95064 USA.
5 : Moss Landing Marine Labs, Moss Landing, CA 95039 USA.
6 : Univ So Calif, Dept Biol Sci, Los Angeles, CA 90089 USA.
7 : Quinault Indian Nation, Div Nat Resources, Dept Fisheries, Taholah, WA 98587 USA.
8 : Olymp Reg Harmful Algal Bloom Partnership, Hoquiam, WA 98550 USA.
9 : IFREMER, Ctr Brest, DYNECO, F-29280 Plouzane, France.
10 : IFREMER, F-29900 Concarneau, France.
Source Journal of Shellfish Research (0730-8000) (National Shellfisheries Association), 2008-12 , Vol. 27 , N. 5 , P. 1301-1310
WOS© Times Cited 28
Keyword(s) Test kit, Razor clams, Scallops, Mussels, ELISA, Domoic acid poisoning, ASP
Abstract Domoic acid (DA) is a potent toxin produced by bloom-forming phytoplankton in the genus which is responsible for causing amnesic shellfish poisoning (ASP) in humans. ASP symptoms include vomiting. diarrhea, and in more severe cases confusion. loss of memory. disorientation. and even coma or death. This paper describes the development and validation of a rapid. sensitive. enzyme linked immunosorbent assay test kit for detecting DA using a monoclonal antibody. The assay gives equivalent results to those obtained using standard high performance liquid chromatography. fluorenylmetbox-yearbonyl high performance liquid chromatography, or liquid chromatography-mass spectrometry methods. It has a linear range from 1.1-3 ppb and was used successfully to measure DA in razor clams, mussels, scallops, and phytoplankton. The assay requires approximately 1.5 h to complete and has a standard 96-well format where each strip of eight wells is removable and can be stored at 4 degrees C until needed. The first two wells of each strip serve as an internal control climinating the need to run a standard curve. This allows as few as 3 or as many as 36 duplicate samples to be run at a time enabling real-time sample processing and limiting degradation of DA. which can occur during storage. There was minimal cross-reactivity in this assay with glutamine, glutamic acid. kainic acid. epi- or iso-DA. This accurate. rapid. cost-effective. assay offers environmental managers and public health officials an effective tool for monitoring DA concentrations in environment samples.
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Litaker R. Wayne, Stewart Thomas N., Eberhart Bich-Thuy L., Wekell John C., Trainer Vera L., Kudela Raphael M., Miller Peter E., Roberts Alice, Hertz Cassandra, Johnson Tyler A., Frankfurter Greg, Smith G. Jason, Schnetzer Astrid, Schumacker Joe, Bastian Jonnette L., Odell Anthony, Gentien Patrick, Le Gal Dominique, Hardison I. Ransom, Tester Patricia A. (2008). Rapid enzyme-linked immunosorbent assay for detection of the algal toxin domoic acid. Journal of Shellfish Research, 27(5), 1301-1310. Open Access version : https://archimer.ifremer.fr/doc/00000/6253/