Molecular cloning, tissue distribution and sequence analysis of complete glucokinase cDNAs from gilthead seabream (Sparus aurata), rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio)

Type Article
Date 2000-03
Language English
Author(s) Panserat Stéphane, Blin C, Medale Francoise, Plagnes Juan E, Breque Jeanine, Krishnamoorthy J, Kaushik Sadasivam
Affiliation(s) INRA, IFREMER, Lab Fish Nutr, F-64310 St Pee Sur Nivelle, France.
Hop Robert Debre, INSERM, U458, F-75019 Paris, France.
Source Biochimica et Biophysica Acta (BBA) - General Subjects (0304-4165) (Elsevier), 2000-03 , Vol. 1474 , N. 1 , P. 61-69
DOI 10.1016/S0304-4165(99)00213-5
WOS© Times Cited 48
Keyword(s) Glucose phosphorylation, Dietary carbohydrate, Fish nutrition
Abstract The enzyme glucokinase (GK) (EC 2.7.1.1) plays an important role in the control of glucose homeostasis. Qualitative and/or quantitative variations in GK enzyme have been postulated by previous studies to explain why dietary carbohydrate utilisation is lower in gilthead seabream (Sparus aurata) and rainbow trout (Oncorhynchus mykiss) than in common carp (Cyprinus carpio). In this study, we report the isolation and characterisation of a full-length cDNA coding for GK in these teleosts. Amino acid sequences derived from these cDNA clones are highly similar to other vertebrate GKs. These findings, including a detailed phylogenetic analysis, reveal that GK gene highly homologous to mammalian GK exists in these fish species with similar tissue specific expression (mainly liver).
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Panserat Stéphane, Blin C, Medale Francoise, Plagnes Juan E, Breque Jeanine, Krishnamoorthy J, Kaushik Sadasivam (2000). Molecular cloning, tissue distribution and sequence analysis of complete glucokinase cDNAs from gilthead seabream (Sparus aurata), rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio). Biochimica et Biophysica Acta (BBA) - General Subjects, 1474(1), 61-69. Publisher's official version : https://doi.org/10.1016/S0304-4165(99)00213-5 , Open Access version : https://archimer.ifremer.fr/doc/00000/669/