||Gagnaire Beatrice1, Frouin Heloise1, 2, Moreau Kevin1, Thomas Guyon Helene2, Renault Tristan1
||1 : IFREMER, LGP, F-17390 La Tremblade, France.
2 : Univ La Rochelle, LBEM, FRE 2727, F-17042 La Rochelle, France.
||Fish & Shellfish Immunology (1050-4648) (Elsevier), 2006-04 , Vol. 20 , N. 4 , P. 536-547
|WOS© Times Cited
||Cellular activity, Flow cytometry, Salinity, Temperature, Haemocyte, Crassostrea gigas, Pacific oyster
||The Pacific oyster, Crassostrea gigas, is extensively cultivated and represents an important economic activity. Oysters are reared in estuarine areas, subjected to various biotic and abiotic factors. One of the limiting factors in aquaculture is mortality outbreaks, which may limit oyster production, and the causes of these outbreaks are not completely understood. In this context, the effects of temperature and salinity on Pacific oyster, C. gigas, haemocytes, were studied. Haemocytes are the invertebrate blood cells and thus have been shown to be involved in defence mechanisms. Flow cytometry was used for monitoring several haemocyte parameters. An increase of temperature induced an increase of haemocyte mortality, in both in vitro and in vivo experiments. Temperature modulated aminopeptidase activity. An in vitro decrease of salinity was associated with cell mortality. During the course of in vivo experiments, an increase of phagocytic activity was reported at 15%. and 50 parts per thousand. Environmental physical parameters may modulate haemocyte activities.