||Fleury Elodie1, Moal Jeanne1, Boulo Viviane2, Daniel Jean-Yves1, Mazurais David, Henaut Alain, Corporeau Charlotte1, Boudry Pierre1, Favrel Pascal1, Huvet Arnaud1
||1 : IFREMER, UMR Nutr Aquaculture & Genom 1067, Ctr Brest, F-29280 Plouzane, France.
2 : Univ Paris 06, CNRS, UMR Systemat 7138, F-75252 Paris, France.
||Marine Biotechnology (1436-2228) (Springer), 2010-06 , Vol. 12 , N. 3 , P. 326-339
|WOS© Times Cited
||Mollusca, Bivalvia, Gene expression, cDNA microarrays, Reproduction, Oxidative stress, Energy metabolism
||Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. Heredity appears to be a major factor determining the sensitivity of oysters to summer mortality, allowing resistant (R) and susceptible (S) lines to be produced. We conducted genome-wide expression profiling of R and S gonads during the 3-month period preceding a summer mortality event, using a cDNA microarray that we designed. ANOVA analysis revealed that 34 genes were differentially expressed between R and S lines on four dates preceding the mortality event. Annotation of some of these genes highlights reproduction and its allocation and antioxidant defenses as the main pathways that operate differentially between R and S lines. This transcriptional analysis provides new indications to define markers for quantitative trait loci searches and functional studies and evaluate the potential role of each gene in the resistance to summer mortality.
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|Author's final draft