Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality

Type Article
Date 2010-06
Language English
Author(s) Fleury ElodieORCID1, Moal Jeanne1, Boulo VivianeORCID2, Daniel Jean-Yves1, Mazurais DavidORCID, Henaut Alain, Corporeau Charlotte1, Boudry PierreORCID1, Favrel Pascal1, Huvet ArnaudORCID1
Affiliation(s) 1 : IFREMER, UMR Nutr Aquaculture & Genom 1067, Ctr Brest, F-29280 Plouzane, France.
2 : Univ Paris 06, CNRS, UMR Systemat 7138, F-75252 Paris, France.
Source Marine Biotechnology (1436-2228) (Springer), 2010-06 , Vol. 12 , N. 3 , P. 326-339
DOI 10.1007/s10126-009-9227-9
WOS© Times Cited 41
Keyword(s) Mollusca, Bivalvia, Gene expression, cDNA microarrays, Reproduction, Oxidative stress, Energy metabolism
Abstract Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. Heredity appears to be a major factor determining the sensitivity of oysters to summer mortality, allowing resistant (R) and susceptible (S) lines to be produced. We conducted genome-wide expression profiling of R and S gonads during the 3-month period preceding a summer mortality event, using a cDNA microarray that we designed. ANOVA analysis revealed that 34 genes were differentially expressed between R and S lines on four dates preceding the mortality event. Annotation of some of these genes highlights reproduction and its allocation and antioxidant defenses as the main pathways that operate differentially between R and S lines. This transcriptional analysis provides new indications to define markers for quantitative trait loci searches and functional studies and evaluate the potential role of each gene in the resistance to summer mortality.
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Fleury Elodie, Moal Jeanne, Boulo Viviane, Daniel Jean-Yves, Mazurais David, Henaut Alain, Corporeau Charlotte, Boudry Pierre, Favrel Pascal, Huvet Arnaud (2010). Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality. Marine Biotechnology, 12(3), 326-339. Publisher's official version : https://doi.org/10.1007/s10126-009-9227-9 , Open Access version : https://archimer.ifremer.fr/doc/00005/11590/