Distribution in Tissue and Seasonal Variation of Norovirus Genogroup I and II Ligands in Oysters

Type Article
Date 2010-08
Language English
Author(s) Maalouf Haifa1, Zakhour Maha2, Le Pendu Jacques2, Le Saux Jean-Claude1, Atmar Robert L.3, Le Guyader Francoise S.1
Affiliation(s) 1 : IFREMER, Microbiol Lab, F-44311 Nantes 03, France.
2 : Univ Nantes, INSERM, U892, Nantes, France.
3 : Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA.
Source Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 2010-08 , Vol. 76 , N. 16 , P. 5621-5630
DOI 10.1128/AEM.00148-10
WOS© Times Cited 78
Abstract Bivalve molluscan shellfish, such as oysters, filter large volumes of water as part of their feeding activities and are able to accumulate and concentrate different types of pathogens, particularly noroviruses, from fecal human pollution. Based on our previous observation of a specific binding of the Norwalk strain (prototype norovirus genogroup I) to the oyster digestive tract through an A-like carbohydrate structure indistinguishable from human blood group A antigen and on the large diversity between strains in terms of carbohydrate-binding specificities, we evaluated the different ligands implicated in attachment to oysters tissues of strains representative of two main genogroups of human norovirus. The GI.1 and GII.4 strains differed in that the latter recognized a sialic acid-containing ligand, present in all tissues, in addition to the A-like ligand of the digestive tract shared with the GI.1 strain. Furthermore, bioaccumulation experiments using wild-type or mutant GI.1 Viruslike particles showed accumulation in hemocytes largely, but not exclusively, based on interaction with the A-like ligand. Moreover, a seasonal effect on the expression of these ligands was detected, most visibly for the GI.1 strain, with a peak in late winter and spring, a period when GI strains are regularly involved in oyster-related outbreaks. These observations may explain some of the distinct epidemiological features of strains from different genogroups.
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Maalouf Haifa, Zakhour Maha, Le Pendu Jacques, Le Saux Jean-Claude, Atmar Robert L., Le Guyader Francoise S. (2010). Distribution in Tissue and Seasonal Variation of Norovirus Genogroup I and II Ligands in Oysters. Applied And Environmental Microbiology, 76(16), 5621-5630. Publisher's official version : https://doi.org/10.1128/AEM.00148-10 , Open Access version : https://archimer.ifremer.fr/doc/00011/12215/