||Rederstorff Emilie1, 2, Fatimi Ahmed2, Sinquin Corinne1, Ratiskol Jacqueline1, Merceron Christophe2, Vinatier Claire2, Weiss Pierre2, Colliec-Jouault Sylvia1
||1 : French Res Inst Exploitat Sea IFREMER, Lab Biotechnol & Marine Mol, F-44311 Nantes 03, France.
2 : Univ Nantes, INSERM UMRS 791, Lab Osteoarticular & Dent Tissue Engn, Sch Dent Surg, F-44042 Nantes 1, France.
||Marine Drugs (1660-3397) (Mdpi Ag), 2011-02 , Vol. 9 , N. 2 , P. 224-241
|WOS© Times Cited
||marine biotechnology, polysaccharides, sterilization procedures, characterization, molecular weight distribution, rheology
||Polysaccharides are highly heat-sensitive macromolecules, so high temperature treatments are greatly destructive and cause considerable damage, such as a great decrease in both viscosity and molecular weight of the polymer. The technical feasibility of the production of exopolysaccharides by deep-sea bacteria Vibrio diabolicus and Alteromonas infernus was previously demonstrated using a bioproduct manufacturing process. The objective of this study was to determine which sterilization method, other than heat sterilization, was the most appropriate for these marine exopolysaccharides and was in accordance with bioprocess engineering requirements. Chemical sterilization using low-temperature ethylene oxide and a mixture of ionized gases (plasmas) was compared to the sterilization methods using gamma and beta radiations. The changes to both the physical and chemical properties of the sterilized exopolysaccharides were analyzed. The use of ethylene oxide can be recommended for the sterilization of polysaccharides as a weak effect on both rheological and structural properties was observed. This low-temperature gas sterilizing process is very efficient, giving a good Sterility Assurance Level (SAL), and is also well suited to large-scale compound manufacturing in the pharmaceutical industry.