Virulence of an emerging pathogenic lineage of Vibrio nigripulchritudo is dependent on two plasmids

Type Article
Date 2011-02
Language English
Author(s) Le Roux Frederique1, 2, 3, Labreuche Yannick4, Davis Brigid M.2, 3, Iqbal Naeem5, 6, Mangenot Sophie7, 8, Goarant Cyrille9, Mazel Didier5, 6, Waldor Matthew K.2, 3, 10
Affiliation(s) 1 : IFREMER, Lab Genet & Pathol, F-3790 La Tremblade, France.
2 : Brigham & Womens Hosp, Channing Lab, Boston, MA 02115 USA.
3 : Harvard Univ, Sch Med, Boston, MA 02115 USA.
4 : IFREMER, Dept Lagons Ecosyst & Aquaculture Durable Nouvell, Noumea 98846, New Caledonia.
5 : Inst Pasteur, Unite Plasticite Genome Bacterien, Dept Genomes & Genet, F-75015 Paris, France.
6 : CNRS, URA2171, F-75015 Paris, France.
7 : CEA, Inst Genom, Evry, France.
8 : CNRS, UMR8030, Lab Genom Comparat, Evry, France.
9 : Inst Pasteur, Noumea, New Caledonia.
10 : HHMI, Boston, MA 02115 USA.
Source Environmental Microbiology (1462-2912) (Wiley-blackwell Publishing, Inc), 2011-02 , Vol. 13 , N. 2 , P. 296-306
DOI 10.1111/j.1462-2920.2010.02329.x
WOS© Times Cited 28
Abstract Vibrioses are the predominant bacterial infections in marine shrimp farms. Vibrio nigripulchritudo is an emerging pathogen of the cultured shrimp Litopenaeus stylirostris in New Caledonia and other regions in the Indo-Pacific. The molecular determinants of V. nigripulchritudo pathogenicity are unknown; however, molecular epidemiological studies have revealed that recent pathogenic V. nigripulchritudo isolates from New Caledonia all cluster into a monophyletic clade and contain a small plasmid, pB1067. Here, we report that a large plasmid, pA1066 (247 kb), can also serve as a marker for virulent V. nigripulchritudo, and that an ancestral version of this plasmid was likely acquired prior to other virulence-linked markers. Additionally, we demonstrate that pA1066 is critical for the full virulence of V. nigripulchritudo in several newly developed experimental models of infection. Plasmid pB1067 also contributes to virulence; only strains containing both plasmids induced the highest level of shrimp mortality. Thus, it appears that these plasmids, which are absent from non-pathogenic isolates, may be driving forces, as well as markers, for the emergence of a pathogenic lineage of V. nigripulchritudo.
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