Relevance of Bacteroidales and F-Specific RNA Bacteriophages for Efficient Fecal Contamination Tracking at the Level of a Catchment in France

Type Article
Date 2012-08
Language English
Author(s) Mauffret Aourell1, Caprais Marie-Paule1, Gourmelon MicheleORCID1
Affiliation(s) 1 : IFREMER, EMP, Microbiol Lab, Plouzane, France.
Source Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 2012-08 , Vol. 78 , N. 15 , P. 5143-5152
DOI 10.1128/AEM.00315-12
WOS© Times Cited 21
Abstract The relevance of three host-associated Bacteroidales markers (HF183, Rum2Bac, and Pig2Bac) and four F-specific RNA bacteriophage genogroups (FRNAPH I to IV) as microbial source tracking markers was assessed at the level of a catchment (Daoulas, France). They were monitored together with fecal indicators (Escherichia coli and enterococci) and chemophysical parameters (rainfall, temperature, salinity, pH, and turbidity) by monthly sampling over 2 years (n = 240 water samples) and one specific sampling following an accidental pig manure spillage (n = 5 samples). During the 2-year regular monitoring, levels of E. coil, enterococci, total F-specific RNA bacteriophages, and the general Bacteroidales marker AllBac were strongly correlated with one another and with Rum2Bac (r = 0.37 to 0.50, P < 0.0001). Their correlations with HF183 and FRNAPH I and II were lower (r = 0.21 to 0.29, P < 0.001 to P < 0.0001), and HF183 and enterococci were associated rather than correlated (Fisher's exact test, P < 0.01). Rum2Bac and FIR 83 enabled 73% of water samples that had >= 2.7 log(10) most probably number (MPN) of E. coli/100 ml to be classified. FRNAPFI I and II enabled 33% of samples at this contamination level to be classified. FRNAPH I and II complemented the water sample classification obtained with the two Bacteroidales markers by an additional 8%. Pig2Bac and FRNAPH III and IV were observed in a small number of samples (n = 0 to 4 of 245). The present study validates Rum2Bac and HF183 as relevant tools to trace fecal contamination originating from ruminant or human waste, respectively, at the level of a whole catchment.
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Mauffret Aourell, Caprais Marie-Paule, Gourmelon Michele (2012). Relevance of Bacteroidales and F-Specific RNA Bacteriophages for Efficient Fecal Contamination Tracking at the Level of a Catchment in France. Applied And Environmental Microbiology, 78(15), 5143-5152. Publisher's official version : https://doi.org/10.1128/AEM.00315-12 , Open Access version : https://archimer.ifremer.fr/doc/00089/20009/