Addressing the measurement of particulate organic and inorganic phosphorus in estuarine and coastal waters

Type Article
Date 2013-06
Language English
Author(s) Labry ClaireORCID1, Youenou Agnes1, Delmas Daniel1, Michelon Pascale1
Affiliation(s) 1 : Ifremer Dyneco Pelagos, F-29280 Plouzane, France.
Source Continental Shelf Research (0278-4343) (Pergamon-elsevier Science Ltd), 2013-06 , Vol. 60 , P. 28-37
DOI 10.1016/j.csr.2013.04.019
WOS© Times Cited 17
Keyword(s) Phosphorus, Speciation, Waters, Phytoplankton, HTC, CWO
Abstract The speciation of particulate phosphorus (TPP) into inorganic (PIP) and organic phosphorus (POP), has been scarcely investigated in suspended particulate matter (SPM), contrary to numerous sediment studies. This work evaluates the actual capacity of methods for TPP, POP and PIP determination in SPM to measure efficiently and specifically each pool. Methods were tested on a range of plankton-associated organic and inorganic P compounds, on microalgal cultures and estuarine samples. Then results are specifically discussed reviewing P speciation in phytoplankton. For TPP analysis, the method of Solorzano and Sharp (1980) is recommended compared with that of Aspila et al. (1976), originally dedicated to sediment, as there is one step less, the protocol is more explicit (use of MgSO4) and it provides the best results. The application of the original protocol of the Aspila method for TPP (no MgSO4) results in underestimated and highly variable values. For POP, the CWO (Chemical Wet Oxidation) method tested here succeeds in providing sufficient oxidation of organic P, but, inorganic polyphosphate is yet too significantly hydrolysed, and intracellular phosphate, an underestimated PIP fraction, contributes to the POP pool with this method. Finally the Aspila method for PIP. is the best compromise in estimating the contribution of PIP to TPP and POP by difference. Even if particulate polyphosphate is only partially hydrolysed (41-49%), intracellular phosphate is included and only a very low percentage of organic P, essentially nucleotidic P, is attacked. In any case, our findings show clear evidence of the necessity to fully describe the procedure when P speciation methods are used.
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