Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers

Type Article
Date 2013-11
Language English
Author(s) Geiger Marie1, 2, Guitton Yann1, Vansteelandt M.1, Kerzaon I.1, 3, Blanchet Estelle1, Du Pont T. Robiou1, Frisvad J. C.4, Hess PhilippORCID2, Pouchus Y. F.1, Grovel Olivier1
Affiliation(s) 1 : Univ Nantes, MMS, F-44035 Nantes, France.
2 : IFREMER, Phycotoxins Lab, Nantes, France.
3 : Univ Lyon 1, UCBL, CNRS, UMR 5557, F-69365 Lyon, France.
4 : Tech Univ Denmark, Ctr Microbial Biotechnol, DK-2800 Lyngby, Denmark.
Source Letters In Applied Microbiology (0266-8254) (Wiley-blackwell), 2013-11 , Vol. 57 , N. 5 , P. 385-392
DOI 10.1111/lam.12143
WOS© Times Cited 14
Keyword(s) cytotoxicity, dereplication, marine fungi, mass spectrometry, Penicillium, Shellfish
Abstract In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish-derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract (Mytilus edulis), mussel flesh-based medium (MES), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host-derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with other constituent identical to YES medium. When shellfish-derived strains of fungi were grown on MES medium, extracts were found to be more cytotoxic than on the YES medium for some of the strains. HPLC-UV/DAD-MS/MS dereplication of extracts from Penicillium marinum and P. restrictum strains grown on MES medium showed the enhancement of the production of some cytotoxic compounds. The mycotoxin patulin was detected in some P. antarcticum extracts, and its presence seemed to be related to their cytotoxicity. Thus, the enhancement of the toxicity of extracts obtained from shellfish-derived Penicillium strains grown on a host-derived medium, and the production of metabolites such as patulin suggests that a survey of mycotoxins in edible shellfish should be considered.
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Geiger Marie, Guitton Yann, Vansteelandt M., Kerzaon I., Blanchet Estelle, Du Pont T. Robiou, Frisvad J. C., Hess Philipp, Pouchus Y. F., Grovel Olivier (2013). Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers. Letters In Applied Microbiology, 57(5), 385-392. Publisher's official version : https://doi.org/10.1111/lam.12143 , Open Access version : https://archimer.ifremer.fr/doc/00160/27166/