Survival, Growth and Reproduction of Cryopreserved Larvae from a Marine Invertebrate, the Pacific Oyster (Crassostrea gigas)

Type Article
Date 2014-04
Language English
Author(s) Suquet Marc1, Labbe Catherine2, Puyo Sophie3, Mingant Christian1, Quittet Benjamin3, Boulais Myrina1, Queau Isabelle1, Ratiskol Dominique1, Diss Blandine4, Haffray Pierrick3
Affiliation(s) 1 : IFREMER, PFOM Dept, Stn Expt Argenton, UMR 6539, Argenton, France.
2 : INRA, LPGP, UR 1037, Rennes, France.
3 : SYSAAF, LPGP, Rennes, France.
4 : Satmar, Barfleur, France.
Source Plos One (1932-6203) (Public Library Science), 2014-04 , Vol. 9 , N. 4 , P. e93486
DOI 10.1371/journal.pone.0093486
WOS© Times Cited 20
Note The present research was supported by the national project CRECHE (Ofimer 136/08/C) and CRYOAQUA (GIS IBISA) and by the European Union (FEP 30906-2009).
Abstract This study is the first demonstration of successful post-thawing development to reproduction stage of diploid cryopreserved larvae in an aquatic invertebrate. Survival, growth and reproductive performances were studied in juvenile and adult Pacific oysters grown from cryopreserved embryos. Cryopreservation was performed at three early stages: trochophore (13 +/- 2 hours post fertilization: hpf), early D-larvae (24 +/- 2 hpf) and late D-larvae (43 +/- 2 hpf). From the beginning (88 days) at the end of the ongrowing phase (195 days), no mortality was recorded and mean body weights did not differ between the thawed oysters and the control. At the end of the growing-out phase (982 days), survival of the oysters cryopreserved at 13 +/- 2 hpf and at 43 +/- 2 hpf was significantly higher (P<0.001) than those of the control (non cryopreserved larvae). Only the batches cryopreserved at 24 +/- 2 hpf showed lower survival than the control. Reproductive integrity of the mature oysters, formely cryopreserved at 13 +/- 2 hpf and 24 +/- 2 hpf, was estimated by the sperm movement and the larval development of their offspring in 13 crosses gamete pools (five males and five females in each pool). In all but two crosses out of 13 tested (P<0.001), development rates of the offspring were not significantly different between frozen and unfrozen parents. In all, the growth and reproductive performances of oysters formerly cryopreserved at larval stages are close to those of controls. Furthermore, these performances did not differ between the three initial larval stages of cryopreservation. The utility of larvae cryopreservation is discussed and compared with the cryopreservation of gametes as a technique for selection programs and shellfish cryobanking.
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Suquet Marc, Labbe Catherine, Puyo Sophie, Mingant Christian, Quittet Benjamin, Boulais Myrina, Queau Isabelle, Ratiskol Dominique, Diss Blandine, Haffray Pierrick (2014). Survival, Growth and Reproduction of Cryopreserved Larvae from a Marine Invertebrate, the Pacific Oyster (Crassostrea gigas). Plos One, 9(4), e93486. Publisher's official version : , Open Access version :