Genetic Inactivation of European Sea Bass (Dicentrarchus labrax L.) Eggs Using UV-Irradiation: Observations and Perspectives

Type Article
Date 2014-10-20
Language English
Author(s) Colleter Julie1, 2, Penman David J.3, Lallement Stephane2, Fauvel Christian2, Hanebrekke Tanja4, Osvik Renate D.4, Eilertsen Hans C.4, D'Cotta Helena1, Chatain Beatrice2, Peruzzi Stefano4
Affiliation(s) 1 : Cirad, Persyst, UMR Intrepid, Montpellier, France.
2 : IFREMER, Lab Rech Piscicole Mediterranee, Stn Expt Aquaculture, Palavas Les Flots, France.
3 : Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland.
4 : Univ Tromso, Fac Biosci Fisheries & Econ, Dept Arctic & Marine Biol, Tromso, Norway.
Source Plos One (1932-6203) (Public Library Science), 2014-10-20 , Vol. 9 , N. 10 , P. 1-11
DOI 10.1371/journal.pone.0109572
WOS© Times Cited 11
Abstract Androgenesis is a form of uniparental reproduction leading to progenies inheriting only the paternal set of chromosomes. It has been achieved with variable success in a number of freshwater species and can be attained by artificial fertilization of genetically inactivated eggs following exposure to gamma (gamma), X-ray or UV irradiation (haploid androgenesis) and by restoration of diploidy by suppression of mitosis using a pressure or thermal shock. The conditions for the genetic inactivation of the maternal genome in the European sea bass (Dicentrarchus labrax L.) were explored using different combinations of UV irradiation levels and durations. UV treatments significantly affected embryo survival and generated a wide range of developmental abnormalities. Despite the wide range of UV doses tested (from 7.2 to 720 mJ.cm(-2)), only one dose (60 mJ.cm(-2).min(-1) with 1 min irradiation) resulted in a small percentage (14%) of haploid larvae at hatching in the initial trials as verified by flow cytometry. Microsatellite marker analyses of three further batches of larvae produced by using this UV treatment showed a majority of larvae with variable levels of paternal and maternal contributions and only one larva displaying pure paternal inheritance. The results are discussed also in the context of an assessment of the UV-absorbance characteristics of egg extracts in this species that revealed the presence of gadusol, a compound structurally related to mycosporine-like amino acids (MAAs) with known UV-screening properties.
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Supporting Information S2 1 38 KB Open access
Supporting Information S3-4-5 2 154 KB Open access
Supporting Information S6 1 20 KB Open access
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Colleter Julie, Penman David J., Lallement Stephane, Fauvel Christian, Hanebrekke Tanja, Osvik Renate D., Eilertsen Hans C., D'Cotta Helena, Chatain Beatrice, Peruzzi Stefano (2014). Genetic Inactivation of European Sea Bass (Dicentrarchus labrax L.) Eggs Using UV-Irradiation: Observations and Perspectives. Plos One, 9(10), 1-11. Publisher's official version : https://doi.org/10.1371/journal.pone.0109572 , Open Access version : https://archimer.ifremer.fr/doc/00239/34996/