During the Sanba (Santos basin seismic transect) experiment in 2010–2011, a 380-km-long combined wide-angle and reflection seismic profile has been acquired using 30 ocean-bottom seismometers, a 4.5 km seismic streamer and a 8900 in.3 airgun array. The Sanba 3 profile crosses the southern flank of the Sao Paulo Plateau, the Sao Paulo Ridge and the easternmost Santos Basin in an east–west direction. Its eastern end is located on undisturbed oceanic crust. Tomographic and forward modelling of the wide-angle seismic data reveals that the sedimentary thickness is variable with only 1–2 km on top of the ridge and thickening to 4–5 km in the basin. Crustal thickness at the ridge is about 18 km and the relative layer thickness and velocity gradients indicate a continental origin of this ridge. The eastern Santos Basin is underlain by crust of only 5 km thickness, characterized by high seismic velocities between 6.20 km s–1 in the upper crust and 7.40 km s–1 in the lower crust. Three hypotheses for the nature of the crust in this region are tested here: (i) thinned continental crust, (ii) serpentinized upper mantle material, (iii) thin oceanic crust. As seismic velocity gradients seem to rule out a continental origin of this region, and clear Moho reflections argue against serpentinized upper mantle, we propose that the crust underlying the easternmost Santos Basin is of oceanic origin. Deviations from normal oceanic crustal velocities in the lower crust (6.70–7.00 km s–1) can be explained by accretion at slow spreading rates leading to the inclusion of serpentinite into the lower crust at the onset of organized seafloor spreading.