Characterization of the Secretomes of Two Vibrios Pathogenic to Mollusks
| Type | Article | ||||||||
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| Date | 2014-11 | ||||||||
| Language | English | ||||||||
| Author(s) | Madec Stephanie1, 5, Pichereau Vianney2, Jacq Annick3, Paillard Mathieu2, 5, Boisset Claire4, 5, Guerard Fabienne2, Paillard Christine2, Nicolas Jean-Louis2, 5 | ||||||||
| Affiliation(s) | 1 : UBO, Lab Univ Biodivers & Ecol Microbienne EA3882, ScInBios SFR48, UEB,ESIAB, F-29280 Plouzane, France. 2 : UBO, UMR UBO CNRS IRD Ifremer 6539, Lab Sci Environm Marin, Inst Univ Europeen Mer, F-29280 Plouzane, France. 3 : Univ Paris 11, CNRS, UMR8621, Inst Genet & Microbiol, F-91405 Orsay, France. 4 : CERMAV CNRS, Ctr Rech Macromol Vegetales, F-38041 Grenoble, France. 5 : Ifremer, France |
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| Source | Plos One (1932-6203) (Public Library Science), 2014-11 , Vol. 9 , N. 11 , P. 1-15 | ||||||||
| DOI | 10.1371/journal.pone.0113097 | ||||||||
| WOS© Times Cited | 13 | ||||||||
| Abstract | Vibrio tapetis causes the brown ring disease in the Japanese clam Ruditapes philippinarum while Vibrio aestuarianus is associated with massive oyster mortalities. As extracellular proteins are often associated with the virulence of pathogenic bacteria, we undertook a proteomic approach to characterize the secretomes of both vibrios. The extracellular proteins (ECPs) of both species were fractionated by SEC-FPLC and in vitro assays were performed to measure the effects of each fraction on hemocyte cellular parameters (phagocytosis and adhesion). Fractions showing a significant effect were subjected to SDS-PAGE, and proteins were identified by nano LC-MS/MS. 45 proteins were identified for V. aestuarianus and 87 for V. tapetis. Most of them belonged to outer membrane or were periplasmic, including porins or adhesins that were already described as virulence factors in other bacterial species. Others were transporter components, flagella proteins, or proteins of unknown function (14 and 15 respectively). Interestingly, for V. aestuarianus, we noted the secretion of 3 extracellular enzymes including the Vam metalloprotease and two other enzymes (one putative lipase and one protease). For V. tapetis, we identified five extracellular enymes, i.e. two different endochitinases, one protease, one lipase and an adhesin. A comparison of both secretomes also showed that only the putative extracellular lipase was common to both secretomes, underscoring the difference in pathogenicity mechanisms between these two species. Overall, these results characterize for the first time the secretomes of these two marine pathogenic vibrios and constitute a useful working basis to further analyze the contribution of specific proteins in the virulence mechanisms of these species. | ||||||||
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