Differentiation of gonyautoxins by ion mobility - mass spectrometry; a cationization study

Type Article
Date 2016-05
Language English
Author(s) Poyer Salome1, 2, Loutelier-Bourhis Corinne1, 2, Tognetti Vincent1, 2, Joubert Laurent1, 2, Enche Julien4, Bossee Anne4, Mondeguer FlorenceORCID3, Hess PhilippORCID3, Afonso Carlos1, 2
Affiliation(s) 1 : Normandie Univ, COBRA, UMR 6014, 1 Rue Tesniere, F-76821 Mont St Aignan, France.
2 : Univ Rouen, INSA Rouen, CNRS, FR 3038,IRCOF, 1 Rue Tesniere, F-76821 Mont St Aignan, France.
3 : IFREMER, Lab Phycotoxines, F-44311 Nantes 03, France.
4 : DGA Maitrise NRBC, Dept Anal Chim, F-91710 Vert Le Petit, France.
Source International Journal Of Mass Spectrometry (1387-3806) (Elsevier Science Bv), 2016-05 , Vol. 402 , P. 20-28
DOI 10.1016/j.ijms.2016.02.013
WOS© Times Cited 7
Keyword(s) Ion mobility, Gonyautoxin, Collision cross-section, Alkali adduct
Abstract Gonyautoxins are potent natural neurotoxic analogues of saxitoxin. Due to their biological activity and submilligram lethal dose for man, fast and efficient methods are required for their characterization. Recent advances in ion mobility-mass spectrometry (IM-MS) showed that differentiation of isomers could be achieved using specific experimental conditions involving particular buffer gases as well as cationic species. In this work, IM-MS experiments were carried out using alkali metal ions (Met = Li+, Na+ or K+) and different buffer gases (N2, N2O and CO2) to improve the differentiation of gonyautoxin isomers. The separation of [GTX + Met]+ ion was achieved for GTX2/3 and GTX1/4 from their Na or K adducts. For dcGTX2/3, the ion mobility separation can only be obtained with peak to peak resolution (Rp-p) close to 1 from the [GTXs + Met − H2O]+ species. To understand the gas phase conformation of the different diastereomers, density functional theory (DFT) calculations were performed. IM separation, and collision cross sections on [GTXs + Met]+ and [GTXs + Met − H2O]+ are reported for the first time.
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Poyer Salome, Loutelier-Bourhis Corinne, Tognetti Vincent, Joubert Laurent, Enche Julien, Bossee Anne, Mondeguer Florence, Hess Philipp, Afonso Carlos (2016). Differentiation of gonyautoxins by ion mobility - mass spectrometry; a cationization study. International Journal Of Mass Spectrometry, 402, 20-28. Publisher's official version : https://doi.org/10.1016/j.ijms.2016.02.013 , Open Access version : https://archimer.ifremer.fr/doc/00317/42863/