Pyrococcus kukulkanii sp nov., a hyperthermophilic, piezophilic archaeon isolated from a deep-sea hydrothermal vent

Type Article
Date 2016-08
Language English
Author(s) Callac Nolwenn1, 2, 3, 4, 5, Oger Philippe6, Lesongeur Francoise1, 2, 3, Rattray Jayne E.5, Vannier Pauline1, 2, 3, Michoud Gregoire1, 2, 3, Beauverger Mickael1, 2, 3, Gayet Nicolas7, Rouxel OlivierORCID8, Jebbar Mohamed1, 2, 3, Godfroy AnneORCID1, 2, 3
Affiliation(s) 1 : Univ Brest, Lab Microbiol Environm Extremes, UEB, IUEM,UMR 6197, 4 Rue Dumont Urville, F-29280 Plouzane, France.
2 : IFREMER, Lab Microbiol Environm Extremes, UMR 6197, Technopole Brest Iroise,CS10070, F-29280 Plouzane, France.
3 : CNRS, Lab Microbiol Environm Extremes, UMR 6197, 4 Rue Dumont Urville, F-29280 Plouzane, France.
4 : Univ Brest, Domaines Ocean IUEM, UMR 6538, 4 Rue Dumont Urville, F-29280 Plouzane, France.
5 : Stockholm Univ, Dept Geol Sci, Stockholm, Sweden.
6 : Univ Lyon, INSA Lyon, CNRS, UMR 5240, Lyon, France.
7 : IFREMER, Lab Environm Profonds, Technopole Brest Iroise,CS10070, F-29280 Plouzane, France.
8 : IFREMER, Lab Geochim & Metallogenie, Technopole Brest Iroise,CS10070, F-29280 Plouzane, France.
Source International Journal Of Systematic And Evolutionary Microbiology (1466-5026) (Microbiology Soc), 2016-08 , Vol. 66 , P. 3142-3149
DOI 10.1099/ijsem.0.001160
WOS© Times Cited 7
Keyword(s) Hyperthermophile, Archaea, Pyrococcus, Piezophile, Thermococcales
Abstract A novel hyperthermophilic, piezophilic, anaerobic archaeon, designated NCB100(T), was isolated from a hydrothermal vent flange fragment collected in the Guaymas basin at the hydrothermal vent site named 'Rebecca's Roost' at a depth of 1997 m. Enrichment and isolation were performed at 100 degrees C under atmospheric pressure. Cells of strain NCB100(T) were highly motile, irregular cocci with a diameter of -1 mu m. Growth was recorded at temperatures between 70 and 112 degrees C (optimum 105 degrees C) and hydrostatic pressures of 0.1-80 MPa (optimum 40-50 MPa). Growth was observed at pH 3.5-8.5 (optimum pH 7) and with 1.5-7% NaCl (optimum at 2.5-3 %). Strain NCB100(T) was a strictly anaerobic chemo-organoheterotroph and grew on complex proteinaceous substrates such as yeast extract, peptone and tryptone, as well as on glycogen and starch. Elemental sulfur was required for growth and was reduced to hydrogen sulfide. The fermentation products from complex proteinaceous substrates were CO2 and H-2. The G+C content of the genomic DNA was 41.3 %. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain NCB100(T) belongs to the genus Pyrococcus, showing 99% similarity with the other described species of the genus Pyrococcus. On the basis of physiological characteristics, DNA G+C content, similarity level between ribosomal proteins and an average nucleotide identity value of 79 %, strain NCB100(T) represents a novel species for which the name Pyrococcus kukulkanii sp. nov. is proposed. The type strain is NCB100(T) (=DSM 101590(T) =Souchotheque de Bretagne BG1337(T)).
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Callac Nolwenn, Oger Philippe, Lesongeur Francoise, Rattray Jayne E., Vannier Pauline, Michoud Gregoire, Beauverger Mickael, Gayet Nicolas, Rouxel Olivier, Jebbar Mohamed, Godfroy Anne (2016). Pyrococcus kukulkanii sp nov., a hyperthermophilic, piezophilic archaeon isolated from a deep-sea hydrothermal vent. International Journal Of Systematic And Evolutionary Microbiology, 66, 3142-3149. Publisher's official version : https://doi.org/10.1099/ijsem.0.001160 , Open Access version : https://archimer.ifremer.fr/doc/00356/46682/