Can the hemoglobin characteristics of vesicomyid clam species influence their distribution in deep-sea sulfide-rich sediments? A case study in the Angola Basin

Type Article
Date 2017-08
Language English
Author(s) Decker Carole1, 2, 3, Zorn N.4, Le Bruchec J.1, Caprais Jean-Claude1, Potier N.4, Leize-Wagner E.4, Lallier F. H.2, 3, Olu Karine1, Andersen A. C.2, 3
Affiliation(s) 1 : IFREMER, Lab Environm Profond, Unite Rech Etud Ecosyst Profonds, F-29280 Plouzane, France.
2 : UPMC Univ Paris 06, UMR 7144, Equipe Adaptat & Biol Invertebres Condit Extremes, Stn Biol, F-29680 Roscoff, France.
3 : CNRS, UMR 7144, Adaptat & Div Milieu Marin, Stn Biol, F-29680 Roscoff, France.
4 : ULP Chim Matiere Complexe, CNRS, UMR 7140, Lab Spectrometrie Masse Interact & Syst, F-67008 Strasbourg, France.
Source Deep-sea Research Part Ii-topical Studies In Oceanography (0967-0645) (Pergamon-elsevier Science Ltd), 2017-08 , Vol. 142 , P. 219-232
DOI 10.1016/j.dsr2.2016.11.009
WOS© Times Cited 14
Keyword(s) Hemoglobin, Mass spectrometry, Oxygen affinity, Symbiont-bearing bivalve, Blood-clams, Cold seeps, Sulfide-rich sediments
Abstract Vesicomyids live in endosymbiosis with sulfur-oxidizing bacteria and therefore need hydrogen sulfide to survive. They can nevertheless live in a wide range of sulfide and oxygen levels and depths, which may explain the exceptional diversity of this clam family in deep-sea habitats. In the Gulf of Guinea, nine species of vesicomyid clams are known to live in cold-seep areas with pockmarks from 600 to 3200 m deep, as well as in the organic-rich sediments of the Congo deep-sea fan at 5000 m deep. Our previous study showed that two species living in a giant pockmark have different oxygen carriers, suggesting different adaptations to hypoxia. Here, we studied the hemoglobin structure and oxygen affinity in three other species, Calyptogena valdiviae, Elenaconcha guiness and Abyssogena southwardae to determine whether the characteristics of their oxygen carriers contribute to their distribution in sulfide-rich sediments at a regional scale. Documenting pairwise species associations in various proportions, we give a semi-quantitative account of their local distribution and oxygen and sulfide measurements at seven sites. Mass spectrometry showed that each vesicomyid species has four intracellular monomeric hemoglobin molecules of 15–16 kDa, all differing in their molecular mass. As expected, the monomers showed no cooperativity in oxygen binding. Their oxygen affinities were very high (below 1 Torr), but differed significantly. C. valdiviae had the highest affinity and was dominant in the Harp pockmark, the site with the lowest oxygen content (half the value of fully oxygenated water). A. southwardae dominated in the Congo Lobe area, the site with the deepest sulfides. We discuss how hemoglobin may favor an active, vertical distribution of vesicomyids in sulfide-rich sediments.
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Decker Carole, Zorn N., Le Bruchec J., Caprais Jean-Claude, Potier N., Leize-Wagner E., Lallier F. H., Olu Karine, Andersen A. C. (2017). Can the hemoglobin characteristics of vesicomyid clam species influence their distribution in deep-sea sulfide-rich sediments? A case study in the Angola Basin. Deep-sea Research Part Ii-topical Studies In Oceanography, 142, 219-232. Publisher's official version : https://doi.org/10.1016/j.dsr2.2016.11.009 , Open Access version : https://archimer.ifremer.fr/doc/00358/46961/