Sunscreen, antioxidant, and bactericide capacities of phlorotannins from the brown macroalga Halidrys siliquosa
| Type | Article | ||||||||||||
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| Date | 2016-12 | ||||||||||||
| Language | English | ||||||||||||
| Author(s) | Le Lann Klervi1, Surget Gwladys1, Couteau Celine2, Coiffard Laurence2, Cerantola Stephane3, Gaillard Fanny4, Larnicol Maud5, Zubia Mayalen6, Guerard Fabienne1, Poupart Nathalie1, Stiger-Pouvreau Valerie1 | ||||||||||||
| Affiliation(s) | 1 : UBO, European Inst Marine Studies IUEM, LEMAR UMR UBO CNRS Ifremer IRD 6539, Technopole Brest Iroise, F-29280 Plouzane, France. 2 : Nantes Atlant Univ, Univ Nantes, Fac Pharm, LPiC,MMS,EA2160, 9 Rue Bias,BP 53508, F-44000 Nantes, France. 3 : UBO, RMN RPE MS, 6 Ave,Victor Le Gorgeu CS93837, F-29238 Brest 3, France. 4 : CNRS, Plateforme Spectrometrie Masse MetaboMER, FR2424, Stn Biol, Pl Georges Teissier,BP 74, F-29682 Roscoff, France. 5 : Venelle Carros, Labs Sci & Mer, CS 70002, F-29480 Le Relecq Kerhuon, France. 6 : Univ Polynesie Francaise, EIO UMR 244, LabEx CORAIL, BP 6570, Faaa 98702, Tahiti, Fr Polynesia. |
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| Source | Journal Of Applied Phycology (0921-8971) (Springer), 2016-12 , Vol. 28 , N. 6 , P. 3547-3559 | ||||||||||||
| DOI | 10.1007/s10811-016-0853-0 | ||||||||||||
| WOS© Times Cited | 63 | ||||||||||||
| Keyword(s) | Antioxidant, Bactericidal, Phenolic compounds, Phaeophyceae, Seaweed, ORAC value, Sunscreen potential | ||||||||||||
| Abstract | The present study focused on a brown macroalga (Halidrys siliquosa), with a particular emphasis on polyphenols and their associated biological activities. Two fractions were obtained by liquid/liquid purification from a crude hydroethanolic extract: (i) an ethyl acetate fraction and (ii) an aqueous fraction. Total phenolic contents and antioxidant activities of extract and both fractions were assessed by in vitro tests (Folin-Ciocalteu test, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power assay, superoxide anion scavenging assay, and beta-carotene-linoleic acid system). For the most active fraction, i.e., the ethyl acetate fraction, the oxygen radical absorbance capacity (ORAC) value, antibacterial activities, and sunscreen potential (Sun Protection Factor and UV-A-Protection Factor) were tested in vitro. A high correlation found between antioxidant activities and total phenolic content was interpreted as the involvement of polyphenolic compounds in antioxidant mechanisms. Interestingly, the ethyl acetate fraction appeared to be a broad-spectrum UV absorber and showed a strong bactericidal activity against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. In this fraction, four phenolic compounds (trifuhalols and tetrafuhalols and, for the first time, diphlorethols and triphlorethols) were identified using 1D and 2D nuclear magnetic resonance (NMR) and MS analysis. These findings are promising for the use of H. siliquosa, abundant in Brittany, as a valuable source of photoprotectant molecules for sunscreen and cosmetic applications. | ||||||||||||
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