Change in the structure of Escherichia coli population and the pattern of virulence genes along a rural aquatic continuum

Type Article
Date 2017-04
Language English
Author(s) Petit Fabienne1, 2, Clermont Olivier3, Delannoy Sabine4, Servais Pierre5, Gourmelon MicheleORCID6, Fach Patrick4, Oberle Kenny1, Fournier Matthieu1, Denamur Erick3, Berthe Thierry1
Affiliation(s) 1 : Normandie Univ, UniRouen, UniCaen, CNRS UMR M2C, Rouen, France.
2 : Sorbonne Univ, CNRS, UPMC, UMR METIS 7619,EPHE, Paris, France.
3 : Univ Paris Diderot, Sorbonne Paris Cite, IAME, INSERM UMR1137, Paris, France.
4 : Univ Paris Est, Anses, Food Safety Lab, IdentyPath Platform, Maisons Alfort, France.
5 : Univ Libre Bruxelles, Ecol Syst Aquat, Campus Plaine, Brussels, Belgium.
6 : RBE SG2M LSEM, Inst Francais Rech Exploitat Mer, Plouzane, France.
Source Frontiers In Microbiology (1664-302X) (Frontiers Media Sa), 2017-04 , Vol. 8 , N. 609 , P. 1-14
DOI 10.3389/fmicb.2017.00609
WOS© Times Cited 7
Keyword(s) water, sediment, pathogenic E. coli, virulence gene, Shiga toxin
Abstract

The aim of this study was to investigate the diversity of the E. coli population, focusing on the occurrence of pathogenic E. coli, in surface water draining a rural catchment. Two sampling campaigns were carried out in similar hydrological conditions (wet period, low flow) along a river continuum, characterized by two opposite density gradients of animals (cattle and wild animals) and human populations. While the abundance of E. coli slightly increased along the river continuum, the abundance of both human and ruminant-associated Bacteroidales markers, as well as the number of E. coli multi-resistant to antibiotics, evidenced a fecal contamination originating from animals at upstream rural sites, and from humans at downstream urban sites. A strong spatial modification of the structure of the E. coli population was observed. At the upstream site close to a forest, a higher abundance of B2 phylogroup strains was observed. At the pasture upstream site, a greater proportion of both E and B1 phylogroups was detected, therefore suggesting a fecal contamination of mainly bovine origin. Conversely, in downstream urban sites, A, D, and F phylogroups were more abundant. To assess the occurrence of intestinal pathogenic strains, virulence factors (afaD, stx1, stx2, eltB (LT), estA (ST), ipaH, bfpA, eae, aaiC and aatA) were screened among 651 E. coli isolates. Intestinal pathogenic strains STEC O174:H21 (stx2) and EHEC O26:H11 (eae, stx1) were isolated in water and sediments close to the pasture site. In contrast, in the downstream urban site aEPEC/EAEC and DAEC of human origin, as well as extra-intestinal pathogenic E. coli belonging to clonal group A of D phylogroup, were sampled. Even if the estimated input of STEC (Shiga toxin-producing E. coli) – released in water at the upstream pasture site – at the downstream site was low, we show that STEC could persist in sediment. These results show that, the run-off of small cattle farms contributed, as much as the wastewater effluent, in the dissemination of pathogenic E. coli in both water and sediments, even if the microbiological quality of the water was good or to average quality according to the French water index.

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How to cite 

Petit Fabienne, Clermont Olivier, Delannoy Sabine, Servais Pierre, Gourmelon Michele, Fach Patrick, Oberle Kenny, Fournier Matthieu, Denamur Erick, Berthe Thierry (2017). Change in the structure of Escherichia coli population and the pattern of virulence genes along a rural aquatic continuum. Frontiers In Microbiology, 8(609), 1-14. Publisher's official version : https://doi.org/10.3389/fmicb.2017.00609 , Open Access version : https://archimer.ifremer.fr/doc/00378/48887/