CO I Barcoding Reveals New Clades and Radiation Patterns of Indo-Pacific Sponges of the Family Irciniidae (Demospongiae: Dictyoceratida)
Type | Article |
---|---|
Date | 2010-04 |
Language | English |
Author(s) | Poeppe Judith1, Sutcliffe Patricia2, Hooper John N. A.2, Woerheide Gert3, 4, Erpenbeck Dirk3, 4 |
Affiliation(s) | 1 : Humboldt Univ, Museum Nat Kunde, Dept Malacozool, Leibniz Inst Res Evolut & Biodivers, Berlin, Germany. 2 : Queensland Museum, Biodivers Program, Brisbane, Australia. 3 : Univ Munich, Dept Earth & Environm Sci Palaeontol & Geobiol, Munich, Germany. 4 : Univ Munich, GeoBioCtr, Munich, Germany. |
Source | Plos One (1932-6203) (Public Library Science), 2010-04 , Vol. 5 , N. 3 , P. e9950 (6p.) |
DOI | 10.1371/journal.pone.0009950 |
WOS© Times Cited | 36 |
Abstract | Background: DNA barcoding is a promising tool to facilitate a rapid and unambiguous identification of sponge species. Demosponges of the order Dictyoceratida are particularly challenging to identify, but are of ecological as well as biochemical importance. Methodology/Principal Findings: Here we apply DNA barcoding with the standard CO1-barcoding marker on selected Indo-Pacific specimens of two genera, Ircinia and Psammocinia of the family Irciniidae. We show that the CO1 marker identifies several species new to science, reveals separate radiation patterns of deep-sea Ircinia sponges and indicates dispersal patterns of Psammocinia species. However, some species cannot be unambiguously barcoded by solely this marker due to low evolutionary rates. Conclusions/Significance: We support previous suggestions for a combination of the standard CO1 fragment with an additional fragment for sponge DNA barcoding. |
Full Text |