The Role of the Glyoxylate Shunt in the Acclimation to Iron Limitation in Marine Heterotrophic Bacteria

Type Article
Date 2018-11
Language English
Author(s) Koedooder Coco1, Gueneugues Audrey1, Van Geersdaele Remy1, Verge Valerie1, Bouget Francois-Yves1, Labreuche Yannick2, 3, Obernosterer Ingrid1, Blain Stephane1
Affiliation(s) 1 : Sorbonne Univ, Lab Oceanog Microbienne, CNRS, Banyuls Sur Mer, France.
2 : Inst Francais Rech Exploitat Mer, Unite Physiol Fonct Organismes Marins, Brest, France.
3 : Sorbonne Univ, CNRS, Integrat Biol Marine Models, Roscoff, France.
Source Frontiers In Marine Science (2296-7745) (Frontiers Media Sa), 2018-11 , Vol. 5 , N. 435 , P. 12p.
DOI 10.3389/fmars.2018.00435
WOS© Times Cited 5
Keyword(s) iron, Fe-limitation, Photobacterium angustum S14, glyoxylate shunt, isocitrate lyase
Abstract

Iron (Fe) is an essential element for marine microbial growth but is present in trace amounts (<0.1 nM) in surface waters of the ocean. In heterotrophic bacteria, Fe-limitation particularly impacts ATP production as Fe is an essential co-factor of enzymes involved in the electron-transport chain as well as the tricarboxylic acid (TCA) cycle. Fe-limitation can therefore drastically reduce both bacterial growth and respiration, consequently affecting the efficiency of organic carbon remineralization. Heterotrophic bacteria possess various strategies to cope with Fe-limitation. In the present study we tested the hypothesis that the induction of the glyoxylate shunt can represent one such strategy. Genetic approaches were used to gain insight into the potential role the glyoxylate shunt may have in alleviating Fe-stress using the gammaproteobacterium, Photobacterium angustum S14. A recombinant bioluminescent reporter of P. angustum S14 (icl-luc) revealed a strong and significant increase in the expression of isocitrate lyase (icl), a key enzyme within the glyoxylate shunt, when cells were subjected to strong Fe-limitation. Although the growth and respiration rates decreased for both the wildtype and an icl knockout mutant (Δicl) under strong Fe-limitation, they were ±30% lower for Δicl as compared to the wildtype. Complementation of Δicl restored the growth and respiration rates to those observed in the wildtype, further confirming the importance of the glyoxylate shunt under strong Fe-limitation. Due to the ubiquitous nature of the glyoxylate shunt within marine bacteria, our results lead us to propose this pathway as an important acclimation strategy for marine heterotrophic bacteria that are subjected to Fe-limitation.

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Koedooder Coco, Gueneugues Audrey, Van Geersdaele Remy, Verge Valerie, Bouget Francois-Yves, Labreuche Yannick, Obernosterer Ingrid, Blain Stephane (2018). The Role of the Glyoxylate Shunt in the Acclimation to Iron Limitation in Marine Heterotrophic Bacteria. Frontiers In Marine Science, 5(435), 12p. Publisher's official version : https://doi.org/10.3389/fmars.2018.00435 , Open Access version : https://archimer.ifremer.fr/doc/00468/58017/