Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly

Type Article
Date 2019-01
Language English
Author(s) Norris Karl1, Mishukova Oksana2, Zykwinska AgataORCID3, Colliec-Jouault SylviaORCID3, Sinquin CorinneORCID3, Koptioug Andrei4, Cuenot Stéphane5, Kerns Jemma6, Surmeneva Maria2, Surmenev Roman2, Douglas Timothy1, 7
Affiliation(s) 1 : Engineering Department, Lancaster University, Lancaster LA1 4YW, UK
2 : Physical Materials Science and Composite Materials Centre, National research Tomsk Polytechnic University, Tomsk 634050, Russia
3 : IFREMER, Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies, F-44311 Nantes, France
4 : Sports Tech Research Centre, Mid-Sweden University, Akademigatan 1, 83125 Östersund, Sweden
5 : Institut des Matériaux Jean Rouxel (IMN), Université de Nantes-CNRS, 44322 Nantes, France
6 : Lancaster Medical School, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YW, UK
7 : Materials Science Institute (MSI), Lancaster University, Lancaster LA1 4YW, UK
Source Micromachines (2072-666X) (MDPI AG), 2019-01 , Vol. 10 , N. 1 , P. 68 (10p.)
DOI 10.3390/mi10010068
WOS© Times Cited 9
Keyword(s) marine exopolysaccharide, collagen, surface modification, Ti6Al4V

Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored. In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization. Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils. Fibril formation resulted in collagen hydrogel formation. Hydrogels formed directly on Ti6Al4V surfaces, and fibrils adsorbed onto the surface. Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils. Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives. Hence, the use of both EPS derivatives as coating components is feasible. Further work should focus on cell differentiation

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Norris Karl, Mishukova Oksana, Zykwinska Agata, Colliec-Jouault Sylvia, Sinquin Corinne, Koptioug Andrei, Cuenot Stéphane, Kerns Jemma, Surmeneva Maria, Surmenev Roman, Douglas Timothy (2019). Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly. Micromachines, 10(1), 68 (10p.). Publisher's official version : , Open Access version :