Inter and intra-specific transcriptional and phenotypic responses of Pseudo-nitzschia under different nutrient conditions

Type Article
Date 2019-03
Language English
Author(s) Lema Anais Kimberley1, Metegnier Gabriel1, 2, Quéré Julien1, Latimier Marie1, Youenou Agnes1, Lambert Christophe3, Fauchot Juliette4, Le Gac MickaelORCID1
Affiliation(s) 1 : IFREMER, Dyneco Pelagos, Plouzané, France
2 : CNRS, Sorbonne Université, Pontificia Universidad Catolica de Chile, Universidad Austral de Chile, UMI 3614, Evolutionary Biology and Ecology of Algae, Station Biologique de Roscoff, Place Georges Teissier, CS90074 29688, Roscoff Cedex, France
3 : Laboratoire des Sciences de l’Environnement Marin (LEMAR), UMR 6539 CNRS UBO IRD IFREMER; Institut Universitaire Européen de la Mer, Technopôle Brest-Iroise, Rue Dumont d’Urville, 29280 Plouzané, France
4 : Normandie Univ, UNICAEN, CNRS, BOREA, Caen, France
Source Genome Biology And Evolution (1759-6653) (Oxford University Press (OUP)), 2019-03 , Vol. 11 , N. 3 , P. 731-747
DOI 10.1093/gbe/evz030
WOS© Times Cited 4
Keyword(s) phenotype, transcriptomics, Pseudo-nitzschia, divergence, nutrients

Untangling the functional basis of divergence between closely related species is a step towards understanding species dynamics within communities at both the evolutionary and ecological scales. We investigated cellular (i.e: growth, domoic acid production, nutrient consumption) and molecular (transcriptomic analyses) responses to varying nutrient concentrations across several strains belonging to three species of the toxic diatom genus Pseudo-nitzschia. Three main results were obtained. First, strains from the same species displayed similar transcriptomic, but not necessarily cellular, responses to the experimental conditions. It showed the importance of considering intra-specific diversity to investigate functional divergence between species. Second, a major exception to the first finding was a strain recently isolated from the natural environment and displaying contrasting gene expression patterns related to cell motility and domoic acid production. This result illustrated the profound modifications that may occur when transferring a cell from the natural to the in vitro environment, and asks for future studies to better understand the influence of culture duration and life cycle on expression patterns. Third, transcriptomic responses were more similar between the two species displaying similar ecology in situ, irrespective of the genetic distance. This was especially true for molecular responses related to TCA cycle, photosynthesis, and nitrogen metabolism. However, transcripts related to phosphate uptake were variable between species. It highlighted the importance of considering both overall genetic distance and ecological divergence to explain functional divergence between species.

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Lema Anais Kimberley, Metegnier Gabriel, Quéré Julien, Latimier Marie, Youenou Agnes, Lambert Christophe, Fauchot Juliette, Le Gac Mickael (2019). Inter and intra-specific transcriptional and phenotypic responses of Pseudo-nitzschia under different nutrient conditions. Genome Biology And Evolution, 11(3), 731-747. Publisher's official version : , Open Access version :