Tissue-Specific Biomarker Responses in the Blue Mussel Mytilus spp. Exposed to a Mixture of Microplastics at Environmentally Relevant Concentrations

Type Article
Date 2019-03
Language English
Author(s) Revel Messika1, Lagarde Fabienne2, Perrein-Ettajani Hanane1, Bruneau Mélanie1, Akcha Farida3, Sussarellu RossanaORCID3, Rouxel Julien3, Costil Katherine4, Decottignies Priscilla5, Cognie Bruno5, Châtel Amélie1, Mouneyrac Catherine1
Affiliation(s) 1 : Laboratoire Mer, Molécules, Santé (MMS EA2160), Université Catholique de l'Ouest, Angers, France
2 : Institut des Molécules et des Matériaux du Mans, UMR CNRS 6283, Le Mans Université, Le Mans, France
3 : Ifremer, Laboratoire d'Ecotoxicologie, Nantes, France
4 : UMR BOREA, MNHN, UPMC, UCBN, CNRS-7208, IRD-207, SFR ICORE, Université de Caen Normandie, Caen, France
5 : FR CNRS 3473 IUML, Laboratoire Mer, Molécules, Santé (MMS EA2160), Université de Nantes, Nantes, France
Source Frontiers In Environmental Science (2296-665X) (Frontiers Media SA), 2019-03 , Vol. 7 , N. 33 , P. 14p.
DOI 10.3389/fenvs.2019.00033
WOS© Times Cited 80
Keyword(s) microplastics, polyethylene, polypropylene, Mytilus, oxidative stress, biomarkers

The impact of a microplastic (MP) mixture composed of polyethylene (PE) and polypropylene (PP) plastic particles, prepared from commercially available products, was evaluated in blue mussels Mytilus spp. exposed to three environmentally relevant concentrations: 0.008 μg L−1 (low), 10 μg L−1 (medium), and 100 μg L−1 (high). Organisms were exposed for 10 days followed by 10 days of depuration in clean seawater under controlled laboratory conditions. The evaluation of MP effects on mussel clearance rate, tissue structure, antioxidant defenses, immune and digestive parameters, and DNA integrity were investigated while the identification of plastic particles in mussel tissues (gills, digestive gland, and remaining tissues), and biodeposits (feces and pseudofaeces) was performed using infrared microscopy (μFT-IR). Results showed the presence of MPs only in the digestive gland of mussels exposed to the highest tested concentration of MPs with a mean of 0.75 particle/mussel (after the 10 days of exposure). In biodeposits, PE and PP particles were detected following exposure to all tested concentrations confirming the ingestion of MPs by the organisms. A differential response of antioxidant enzyme activities between digestive gland and gills was observed. Significant increases in superoxide dismutase (SOD) and catalase (CAT) activities were measured in the digestive gland of mussels exposed to the low (0.008 μg L−1) and medium (10 μg L−1) concentrations of MPs and in the gills from mussels exposed to the highest concentration (100 μg L−1) of MPs that could be indicative of a change in the redox balance. Moreover, an increase in acid phosphatase activity was measured in hemolymph of mussels exposed to 0.008 and 10 μg L−1 concentrations. No significant difference was observed in the clearance rate, and histopathological parameters between control and exposed mussels. This study brings new insights on the potential sublethal impacts of MPs at environmentally relevant concentrations in marine bivalves.

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Revel Messika, Lagarde Fabienne, Perrein-Ettajani Hanane, Bruneau Mélanie, Akcha Farida, Sussarellu Rossana, Rouxel Julien, Costil Katherine, Decottignies Priscilla, Cognie Bruno, Châtel Amélie, Mouneyrac Catherine (2019). Tissue-Specific Biomarker Responses in the Blue Mussel Mytilus spp. Exposed to a Mixture of Microplastics at Environmentally Relevant Concentrations. Frontiers In Environmental Science, 7(33), 14p. Publisher's official version : https://doi.org/10.3389/fenvs.2019.00033 , Open Access version : https://archimer.ifremer.fr/doc/00489/60089/