It has been shown that the deep Eastern Equatorial Pacific (EEP) region was poorly ventilated during the Last Glacial Maximum (LGM) relative to Holocene values. This finding suggests a more efficient biological pump, which indirectly supports the idea of increased carbon storage in the deep ocean contributing to lower atmospheric CO2 during the last glacial. However, proxies related to respired carbon are needed in order to directly test this proposition. Here we present Cibicides wuellerstorfi B/Ca ratios from Ocean Drilling Program Site 1240 measured by laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) as a proxy for deep water carbonate saturation state ([CO32-], and therefore [CO32-]), along with C-13 measurements. In addition, the U/Ca ratio in foraminiferal coatings has been analyzed as an indicator of oxygenation changes. Our results show lower [CO32-], C-13, and [O-2] values during the LGM, which would be consistent with higher respired carbon levels in the deep EEP driven, at least in part, by reduced deep water ventilation. However, the difference between LGM and Holocene [CO32-] observed at our site is relatively small, in accordance with other records from across the Pacific, suggesting that a counteracting mechanism, such as seafloor carbonate dissolution, also played a role. If so, this mechanism would have increased average ocean alkalinity, allowing even more atmospheric CO2 to be sequestered by the ocean. Therefore, the deep Pacific Ocean very likely stored a significant amount of atmospheric CO2 during the LGM, specifically due to a more efficient biological carbon pump and also an increase in average ocean alkalinity.