Photochemistry on the Space Station-Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station

Type Article
Date 2019-08
Language English
Author(s) Coussot Gaelle1, Le Postollec Aurelie2, Incerti SebastienORCID3, Baque MickaelORCID4, Faye Clement5, Vandenabeele Trambouze Odile6, Cottin HerveORCID7, 8, Ravelet Corinne9, Peyrin Eric9, Fiore Emmanuelle9, Vigier Flavie, Caron Jerome10, Chaput Didier11, Przybyla Bartos12, Berger ThomasORCID12, Dobrijevic Michel2
Affiliation(s) 1 : Univ Montpellier, ENSCM, CNRS, IBMM, F-34093 Montpellier 5, France.
2 : Univ Bordeaux, B18N, CNRS, LAB, Pessac, France.
3 : Univ Bordeaux, UMR 5797, CENBG, Gradignan, France.
4 : German Aerosp Ctr DLR, Inst Planetary Res Management & Infrastruct, Res Grp Astrobiol Labs, Berlin, Germany.
5 : Colcom, Cap Alpha, Clapiers, France.
6 : UBO, LMEE, IUEM UMR 6197, Plouzane, France.
7 : Univ Paris Est Creteil, UMR 7583, LISA, Creteil, France.
8 : Univ Paris Diderot, Inst Pierre Simon Lapl, Creteil, France.
9 : Univ Grenoble Alpes, Dept Pharmacochim Mol, CNRS, UMR 5063, St Martin Dheres, France.
10 : Comprehens Canc Ctr, Inst Bergonie, Dept Radiotherapie, Bordeaux, France.
11 : Ctr Natl Etud Spatiales, DCT ME EM, Toulouse, France.
12 : German Aerosp Ctr, Inst Aerosp Med, Cologne, Germany.
Source Astrobiology (1531-1074) (Mary Ann Liebert, Inc), 2019-08 , Vol. 19 , N. 8 , P. 1063-1074
DOI 10.1089/ast.2018.1896
WOS© Times Cited 3
Keyword(s) Astrobiology, Cosmic rays, Biochip, Aptamers
Abstract

Some microarray-based instruments that use bioaffinity receptors such as antibodies or aptamers are under development to detect signatures of past or present life on planetary bodies. Studying the resistance of such instruments against space constraints and cosmic rays in particular is a prerequisite. We used several ground-based facilities to study the resistance of aptamers to various types of particles (protons, electrons, neutrons, and carbon ions) at different energies and fluences. We also tested the resistance of aptamers during the EXPOSE-R2 mission outside the International Space Station (ISS). The accumulated dose measured after the 588 days of this mission (220 mGy) corresponds to the accumulated dose that can be expected during a mission to Mars. We found that the recognition ability of fluorescently labeled aptamers was not significantly affected during short-term exposure experiments taking into account only one type of radiation at a time. However, we demonstrated that the same fluorescent dye was significantly affected by temperature variations (-21 degrees C to +58 degrees C) and storage throughout the entirety of the ISS experiment (60% of signal loss). This induced a large variability of aptamer signal in our analysis. However, we found that >50% of aptamers were still functional after the whole EXPOSE-R2 mission. We conclude that aptamer-based instruments are well suited for in situ analysis on planetary bodies, but the detection step requires additional investigations.

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Coussot Gaelle, Le Postollec Aurelie, Incerti Sebastien, Baque Mickael, Faye Clement, Vandenabeele Trambouze Odile, Cottin Herve, Ravelet Corinne, Peyrin Eric, Fiore Emmanuelle, Vigier Flavie, Caron Jerome, Chaput Didier, Przybyla Bartos, Berger Thomas, Dobrijevic Michel (2019). Photochemistry on the Space Station-Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station. Astrobiology, 19(8), 1063-1074. Publisher's official version : https://doi.org/10.1089/ast.2018.1896 , Open Access version : https://archimer.ifremer.fr/doc/00601/71306/