Species specific gene expression dynamics during harmful algal blooms

Type Article
Date 2020-04
Language English
Author(s) Metegnier Gabriel1, 2, Paulino Sauvann1, Ramond Pierre1, 3, Siano Raffaele1, Sourisseau Marc1, Destombe Christophe2, Le Gac MickaelORCID1
Affiliation(s) 1 : French Research Institute for Exploitation of the Sea, Ifremer DYNECO PELAGOS, 29280, Plouzané, France
2 : CNRS, Sorbonne Université, UC, UaCh, UMI 3614, Evolutionary Biology and Ecology of Algae, Station Biologique de Roscoff, CS 90074, 29688, Roscoff, France
3 : CNRS, Sorbonne Université, UMR 7144, Station Biologique de Roscoff, CS90074, 29688, Roscoff Cedex, France
Source Scientific Reports (2045-2322) (Springer Science and Business Media LLC), 2020-04 , Vol. 10 , N. 1 , P. 6182 (14p.)
DOI 10.1038/s41598-020-63326-8

Harmful algal blooms are caused by specific members of microbial communities. Understanding the dynamics of these events requires comparing the strategies developed by the problematic species to cope with environmental fluctuations to the ones developed by the other members of the community. During three consecutive years, the meta-transcriptome of micro-eukaryote communities was sequenced during blooms of the toxic dinoflagellate Alexandrium minutum. The dataset was analyzed to investigate species specific gene expression dynamics. Major shifts in gene expression were explained by the succession of different species within the community. Although expression patterns were strongly correlated with fluctuation of the abiotic environment, and more specifically with nutrient concentration, transcripts specifically involved in nutrient uptake and metabolism did not display extensive changes in gene expression. Compared to the other members of the community, A. minutum displayed a very specific expression pattern, with lower expression of photosynthesis transcripts and central metabolism genes (TCA cycle, glucose metabolism, glycolysis…) and contrasting expression pattern of ion transporters across environmental conditions. These results suggest the importance of mixotrophy, cell motility and cell-to-cell interactions during A. minutum blooms.

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