New Insight into Antimicrobial Compounds from Food and Marine-Sourced Carnobacterium Species through Phenotype and Genome Analyses

Type Article
Date 2020-07
Language English
Author(s) Begrem Simon1, 2, Ivaniuk Flora2, Gigout Chevalier FrederiqueORCID2, Kolypczuk LaetitiaORCID2, Bonnetot Sandrine2, Leroi Francoise2, Grovel Olivier1, Delbarre-Ladrat ChristineORCID2, Passerini DelphineORCID2
Affiliation(s) 1 : University of Nantes, 44035 Nantes CEDEX 1, France
2 : IFREMER, BRM, EM3B Laboratory, 44300 Nantes CEDEX 3, France
Source Microorganisms (2076-2607) (MDPI AG), 2020-07 , Vol. 8 , N. 7 , P. 1093 (21p.)
DOI 10.3390/microorganisms8071093
Note This article belongs to the Special Issue Screening and Characterization of the Diversity of Food Microorganisms and Their Metabolites
Keyword(s) lactic acid bacteria, antimicrobial activity, Carnobacteriumspp, hydrogen peroxide, bacteriocin, RiPP, NRPS, terpene, natural product, genome mining
Abstract

Carnobacterium maltaromaticum and Carnobacterium divergens, isolated from food products, are lactic acid bacteria known to produce active and efficient bacteriocins. Other species, particularly those originating from marine sources, are less studied. The aim of the study is to select promising strains with antimicrobial potential by combining genomic and phenotypic approaches on large datasets comprising 12 Carnobacterium species. The biosynthetic gene cluster (BGCs) diversity of 39 publicly available Carnobacterium spp. genomes revealed 67 BGCs, distributed according to the species and ecological niches. From zero to six BGCs were predicted per strain and classified into four classes: terpene, NRPS (non-ribosomal peptide synthetase), NRPS-PKS (hybrid non-ribosomal peptide synthetase-polyketide synthase), RiPP (ribosomally synthesized and post-translationally modified peptide). In parallel, the antimicrobial activity of 260 strains from seafood products was evaluated. Among the 60% of active strains, three genomes were sequenced and submitted to a dereplication process. C. inhibens MIP2551 produced a high amountof H2O2, probably thanks to the presence of four oxidase-encoding genes. C. maltaromaticum EBP3019 and SF668 strains were highly efficient against Listeria monocytogenes. A new extracellular 16 kDa unmodified bacteriocin in the EBP3019 strain and five different bacteriocins in SF668 were highlighted. In this study, the overview of antimicrobial BGC and inhibitory activities of Carnobacterium spp. allowed the prediction of potential innovative natural products that could be relevant for biotechnological applications

Full Text
File Pages Size Access
Publisher's official version 21 1 MB Open access
Supplementary File 1 187 KB Open access
Top of the page

How to cite 

Begrem Simon, Ivaniuk Flora, Gigout Chevalier Frederique, Kolypczuk Laetitia, Bonnetot Sandrine, Leroi Francoise, Grovel Olivier, Delbarre-Ladrat Christine, Passerini Delphine (2020). New Insight into Antimicrobial Compounds from Food and Marine-Sourced Carnobacterium Species through Phenotype and Genome Analyses. Microorganisms, 8(7), 1093 (21p.). Publisher's official version : https://doi.org/10.3390/microorganisms8071093 , Open Access version : https://archimer.ifremer.fr/doc/00641/75335/