An environmentally relevant mixture of polychlorinated biphenyls (PCBs) and polybrominated diphenylethers (PBDEs) disrupts mitochondrial function, lipid metabolism and neurotransmission in the brain of exposed zebrafish and their unexposed F2 offspring

Type Article
Date 2021-02
Language English
Author(s) Blanc Mélanie1, Alfonso Sebastien2, 3, Bégout Marie-LaureORCID2, Barrachina Célia4, Hyötyläinen Tuulia1, Keiter Steffen H.1, Cousin XavierORCID2, 5
Affiliation(s) 1 : Man-Technology-Environment Research Centre (MTM), School of Science and Technology, Örebro University, Fakultetsgatan 1, S-701 82 Örebro, Sweden
2 : MARBEC, Univ. Montpellier, CNRS, Ifremer, IRD, Route de Maguelone, F-34250 Palavas-les-Flots, France
3 : COISPA Tecnologia & Ricerca, Stazione Sperimentale per lo Studio delle Risorse del Mare, Via dei Trulli, n 18, 70126 Bari, Italy
4 : MGX, Univ. Montpellier, CNRS, INSERM, Université Montpellier 2, Place Eugène Bataillon, F-34095 Montpellier, France
5 : Université Paris-Saclay, AgroParisTech, INRAE, GABI, Domaine de Vilvert, F-78350 Jouy-en-Josas, France
Source Science of The Total Environment (00489697) (Elsevier BV), 2021-02 , Vol. 754 , P. 142097 (9p.)
DOI 10.1016/j.scitotenv.2020.142097
Keyword(s) Danio rerio, persistent organic pollutant, neurotoxicity, brain metabolism, RNA-seq
Abstract

Polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants still present in aquatic environments despite their total or partial ban. Previously, we observed that an environmentally realistic mixture of these compounds affects energy balance, growth, and reproduction in exposed zebrafish (F0), and behavior in their unexposed offspring (F1-F4). In the present work, we performed lipidomic and transcriptomic analyses on brains of zebrafish (F0-F2) from exposed and control lineages to identify molecular changes that could explain the observed phenotypes. The use of both technologies highlighted that F0 zebrafish displayed impaired mitochondrial function and lipid metabolism regulation (depletion in triacylglycerols and phospholipids) which can explain disruption of energy homeostasis. A subset of the regulated biological pathways related to energetic metabolism and neurotransmission were inherited in F2. In addition, there were increasing effects on epigenetic pathways from the F0 to the F2 generation. Altogether, we show that the effects of an environmental exposure to PCBs and PBDEs on energetic metabolism as well as neurotransmission extend over 2 generations of zebrafish, possibly due to transgenerational epigenetic inheritance.

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Blanc Mélanie, Alfonso Sebastien, Bégout Marie-Laure, Barrachina Célia, Hyötyläinen Tuulia, Keiter Steffen H., Cousin Xavier (2021). An environmentally relevant mixture of polychlorinated biphenyls (PCBs) and polybrominated diphenylethers (PBDEs) disrupts mitochondrial function, lipid metabolism and neurotransmission in the brain of exposed zebrafish and their unexposed F2 offspring. Science of The Total Environment, 754, 142097 (9p.). Publisher's official version : https://doi.org/10.1016/j.scitotenv.2020.142097 , Open Access version : https://archimer.ifremer.fr/doc/00646/75797/