Measuring Histone Modifications in the Human Parasite Schistosoma mansoni.
Type | Book section | ||||||||||||
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Date | 2020 | ||||||||||||
Language | English | ||||||||||||
Author(s) | de Carvalho Augusto Ronaldo1, 2, Roquis David3, Al Picard Marion4, Chaparro Christian1, Cosseau Celine1, Grunau Christoph1 | ||||||||||||
Affiliation(s) | 1 : IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia Perpignan , France 2 : LBMC, Laboratoire de Biologie et Modélisation de la Cellule Univ Lyon, ENS de Lyon Lyon, France 3 : Amélioration des Grandes Cultures et Ressources Génétiques, Agroscope Changins, Switzerland 4 : Centre National de la Recherche Scientifique (CNRS), Laboratory MIVEGEC (CNRS IRD Uni Montpellier) Montpellier, France |
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Book | imson D.J. (eds) Schistosoma mansoni. Methods in Molecular Biology, vol 2151. Print ISBN 978-1-0716-0634-6 Online ISBN 978-1-0716-0635-3. Chap.9, pp.93-107 | ||||||||||||
DOI | 10.1007/978-1-0716-0635-3_9 | ||||||||||||
Keyword(s) | Native chromatin immunoprecipitation, ChIPmentation, Histone modifications, Schistosomiasis, Trematode, Development | ||||||||||||
Abstract | DNA-binding proteins play critical roles in many major processes such as development and sexual biology of Schistosoma mansoni and are important for the pathogenesis of schistosomiasis. Chromatin immunoprecipitation (ChIP) experiments followed by sequencing (ChIP-seq) are useful to characterize the association of genomic regions with posttranslational chemical modifications of histone proteins. Challenges in the standard ChIP protocol have motivated recent enhancements in this approach, such as reducing the number of cells required and increasing the resolution. In this chapter, we describe the latest advances made by our group in the ChIP methods to improve the standard ChIP protocol to reduce the number of input cells required and to increase the resolution and robustness of ChIP in S. mansoni. |
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