Enhancing PUFA-rich polar lipids in Tisochrysis lutea using adaptive laboratory evolution (ALE) with oscillating thermal stress

Type Article
Date 2021-01
Language English
Author(s) Gachelin Manon1, Boutoute Marc1, Carrier GregoryORCID2, Talec Amélie1, Pruvost Eric1, Guihéneuf Freddy1, 3, Bernard Olivier4, Sciandra Antoine1
Affiliation(s) 1 : Laboratoire d’Océanographie de Villefranche (LOV, UMR 7093), Sorbonne Universités, CNRS, Station zoologique, 181 Chemin du Lazaret, 06230, Villefranche-sur-Mer, France
2 : Laboratoire Physiologie et Biotechnologie des Algues (PBA), IFREMER, Nantes, France
3 : SAS Inalve, 181 chemin du lazaret, 06230, Villefranche-sur-Mer, France
4 : Biocore, INRIA Sophia Antipolis Méditerranée, Valbonne, France
Source Applied Microbiology And Biotechnology (0175-7598) (Springer Science and Business Media LLC), 2021-01 , Vol. 105 , N. 1 , P. 301-312
DOI 10.1007/s00253-020-11000-4
WOS© Times Cited 15
Keyword(s) Tisochrysis lutea, Polar lipids, Adaptive laboratory evolution (ALE), Temperature, DHA

Adaptive laboratory evolution is a powerful tool for microorganism improvement likely to produce enhanced microalgae better tailored to their industrial uses. In this work, 12 wild-type strains of Tisochrysis lutea were co-cultivated under increasing thermal stress for 6 months. Indeed, temperature was oscillating daily between a high and a low temperature, with increasing amplitude along the experiment. The goal was to enhance the polyunsaturated fatty acid content of the polar lipids. Samples were taken throughout the evolution experiment and cultivated in standardized conditions to analyze the evolution of the lipid profile. Genomic analysis of the final population shows that two strains survived. The lipid content doubled, impacting all lipid classes. The fatty acid analyses show a decrease in SFAs correlated with an increase in monounsaturated fatty acids (MUFAs), while changes in polyunsaturated fatty acid (PUFAs) vary between both photobioreactors. Hence, the proportion of C18-MUFAs (18:1 n-9) and most C18-PUFAs (18:2 n-6, 18:3 n-3, and 18:4 n-3) increased, suggesting their potential role in adjusting membrane fluidity to temperature shifts. Of particular interest, DHA in polar lipids tripled in the final population while the growth rate was not affected

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Gachelin Manon, Boutoute Marc, Carrier Gregory, Talec Amélie, Pruvost Eric, Guihéneuf Freddy, Bernard Olivier, Sciandra Antoine (2021). Enhancing PUFA-rich polar lipids in Tisochrysis lutea using adaptive laboratory evolution (ALE) with oscillating thermal stress. Applied Microbiology And Biotechnology, 105(1), 301-312. Publisher's official version : https://doi.org/10.1007/s00253-020-11000-4 , Open Access version : https://archimer.ifremer.fr/doc/00661/77269/