Bacteria enhance the production of extracellular polymeric substances by the green dinoflagellate Lepidodinium chlorophorum

Type Article
Date 2021-12
Language English
Author(s) Roux Pauline1, Siano Raffaele2, Collin Karine1, Bilien GwenaelORCID3, Sinquin CorinneORCID4, Marchand Laetitia4, Zykwinska AgataORCID4, Delbarre-Ladrat ChristineORCID4, Schapira Mathilde1
Affiliation(s) 1 : Ifremer, LITTORAL, 44300, Nantes, France
2 : Ifremer, DYNECO, 29280, Plouzané, France
3 : Ifremer, LITTORAL, 29900, Concarneau, France
4 : Ifremer, BRM, 44300, Nantes, France
Source Scientific Reports (2045-2322) (Springer Science and Business Media LLC), 2021-12 , Vol. 11 , N. 1 , P. 4795 (15p.)
DOI 10.1038/s41598-021-84253-2
Keyword(s) Bacteria, Biogeochemistry, Carbohydrates, Community ecology, Ecology, Ecophysiology, Environmental sciences, Microbial ecology, Ocean sciences, Water microbiology
Abstract

High biomasses of the marine dinoflagellate Lepidodinium chlorophorum cause green seawater discolorations along Southern Brittany (NE Atlantic, France). The viscosity associated to these phenomena has been related to problems in oyster cultivation. The harmful effect of L. chlorophorum might originate from the secretion of Extracellular Polymeric Substances (EPS). To understand whether the EPS are produced by L. chlorophorum or its associated bacteria, or if they are a product of their interaction, batch cultures were performed under non-axenic and pseudo-axenic conditions for three strains. Maximum dinoflagellate cell abundances were observed in pseudo-axenic cultures. The non-sinking fraction of polymers (Soluble Extracellular Polymers, SEP), mainly composed of proteins and the exopolysaccharide sulphated galactan, slightly increased in pseudo-axenic cultures. The amount of Transparent Exopolymer Particles (TEP) per cell increased under non-axenic conditions. Despite the high concentrations of Particulate Organic Carbon (POC) measured, viscosity did not vary. These results suggest that the L. chlorophorum-bacteria interaction could have a detrimental consequence on the dinoflagellate, translating in a negative effect on L. chlorophorum growth, as well as EPS overproduction by the dinoflagellate, at concentrations that should not affect seawater viscosity.

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How to cite 

Roux Pauline, Siano Raffaele, Collin Karine, Bilien Gwenael, Sinquin Corinne, Marchand Laetitia, Zykwinska Agata, Delbarre-Ladrat Christine, Schapira Mathilde (2021). Bacteria enhance the production of extracellular polymeric substances by the green dinoflagellate Lepidodinium chlorophorum. Scientific Reports, 11(1), 4795 (15p.). Publisher's official version : https://doi.org/10.1038/s41598-021-84253-2 , Open Access version : https://archimer.ifremer.fr/doc/00682/79403/