Functional Insights From the Evolutionary Diversification of Big Defensins

Type Article
Date 2020-04
Language English
Author(s) Gerdol Marco1, Schmitt Paulina2, Venier PaolaORCID3, Rocha Gustavo4, Rosa Rafael DiegoORCID4, Destoumieux-Garzon DelphineORCID5
Affiliation(s) 1 : Univ Trieste, Dept Life Sci, Trieste, Italy.
2 : Pontificia Univ Catolica Valparaiso, Inst Biol, Lab Genet & Inmunol Mol, Valparaiso, Chile.
3 : Univ Padua, Dept Biol, Padua, Italy.
4 : Univ Fed Santa Catarina, Dept Cell Biol Embryol & Genet, Lab Immunol Appl Aquaculture, Florianopolis, SC, Brazil.
5 : Univ Perpignan, Univ Montpellier, CNRS, IHPE, Via Domitia, Montpellier, France.
Source Frontiers In Immunology (1664-3224) (Frontiers Media Sa), 2020-04 , Vol. 11 , N. 758 , P. 16p.
DOI 10.3389/fimmu.2020.00758
WOS© Times Cited 6
Keyword(s) antimicrobial peptide, defensin, evolution, nanonet, host defense (antimicrobial) peptides
Abstract Big defensins are antimicrobial polypeptides believed to be the ancestors of beta-defensins, the most evolutionary conserved family of host defense peptides (HDPs) in vertebrates. Nevertheless, big defensins underwent several independent gene loss events during animal evolution, being only retained in a limited number of phylogenetically distant invertebrates. Here, we explore the evolutionary history of this fascinating HDP family and investigate its patchy distribution in extant metazoans. We highlight the presence of big defensins in various classes of lophotrochozoans, as well as in a few arthropods and basal chordates (amphioxus), mostly adapted to life in marine environments. Bivalve mollusks often display an expanded repertoire of big defensin sequences, which appear to be the product of independent lineage-specific gene tandem duplications, followed by a rapid molecular diversification of newly acquired gene copies. This ongoing evolutionary process could underpin the simultaneous presence of canonical big defensins and non-canonical (beta-defensin-like) sequences in some species. The big defensin genes of mussels and oysters, two species target of in-depth studies, are subjected to gene presence/absence variation (PAV), i.e., they can be present or absent in the genomes of different individuals. Moreover, big defensins follow different patterns of gene expression within a given species and respond differently to microbial challenges, suggesting functional divergence. Consistently, current structural data show that big defensin sequence diversity affects the 3D structure and biophysical properties of these polypeptides. We discuss here the role of the N-terminal hydrophobic domain, lost during evolution toward beta-defensins, in the big defensin stability to high salt concentrations and its mechanism of action. Finally, we discuss the potential of big defensins as markers for animal health and for the nature-based design of novel therapeutics active at high salt concentrations.
Full Text
File Pages Size Access
Publisher's official version 16 4 MB Open access
Data Sheet 1 426 KB Open access
Data Sheet 2 1 MB Open access
Table 1 39 KB Open access
Top of the page