Development in the European flounder (Platichthys flesus) of a q-PCR assay for the measurement of telomere length, a potential biomarker of pollutant effects for biomonitoring studies

Type Article
Date 2021-09
Language English
Author(s) Akcha Farida1, Cahuc C.1, Rouxel Julien1, Munschy Catherine1, Aminot YannORCID1, Chouvelon Tiphaine1, Mahe KeligORCID2, Budzinski H.3, Mauffret AourellORCID1
Affiliation(s) 1 : Ifremer, Biogeochemistry and Ecotoxicology Research Unit, Nantes, France
2 : Ifremer, Laboratory of Fisheries Resources, Boulogne-Sur-Mer, France
3 : UMR CNRS 5805 EPOC, Laboratory of Physico- and Toxico-Chemistry of the Environment, Bordeaux, France
Source Marine Pollution Bulletin (0025-326X) (Elsevier BV), 2021-09 , Vol. 170 , P. 112610 (13p.)
DOI 10.1016/j.marpolbul.2021.112610
Keyword(s) Flatfish, Pollution, Biomonitoring, Telomere, Genotoxicity, DNA damage
Abstract

Telomeres protect the coding sequence of chromosome ends and Telomere Length (TL) has been proposed as a biomarker of cellular aging, cumulative stress exposure and life-span in humans. With the aim to propose new biomarkers, a q-PCR protocol was adapted for the measurement of TL in the European flounder Platichthys flesus. The protocol was then applied in 2-year-old flounders from the Seine Estuary.

The absolute TL in the flounder is 54 ± 13 kbp per genome (mean ± standard error). Considering relative or absolute TL, no correlation was observed with DNA damage and any of the measured contaminant concentrations (trace elements, metabolites of polycyclic aromatic hydrocarbons, polychlorobiphenyls, organochlorinated pesticides, polybrominated diphenyl ethers, perfluoroalkyl substances). Because sampling was limited, further investigations are required to state a possible impact of chemical pollution on flatfish telomeres. This is motivated by correlations observed with organochlorinated compounds when decreasing statistical significance (p ≤ 0.10).

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Akcha Farida, Cahuc C., Rouxel Julien, Munschy Catherine, Aminot Yann, Chouvelon Tiphaine, Mahe Kelig, Budzinski H., Mauffret Aourell (2021). Development in the European flounder (Platichthys flesus) of a q-PCR assay for the measurement of telomere length, a potential biomarker of pollutant effects for biomonitoring studies. Marine Pollution Bulletin, 170, 112610 (13p.). Publisher's official version : https://doi.org/10.1016/j.marpolbul.2021.112610 , Open Access version : https://archimer.ifremer.fr/doc/00700/81161/